Numbers in parenthesis represent values for the highest resolution bin.
The crystal structure of the ING5 PHD finger in complex with an H3K4me3 histone peptide
Article first published online: 11 JUL 2008
Copyright © 2008 Wiley-Liss, Inc.
Proteins: Structure, Function, and Bioinformatics
Volume 72, Issue 4, pages 1371–1376, September 2008
How to Cite
Champagne, K. S., Saksouk, N., Peña, P. V., Johnson, K., Ullah, M., Yang, X.-J., Côté, J. and Kutateladze, T. G. (2008), The crystal structure of the ING5 PHD finger in complex with an H3K4me3 histone peptide. Proteins, 72: 1371–1376. doi: 10.1002/prot.22140
- Issue published online: 24 JUL 2008
- Article first published online: 11 JUL 2008
- Manuscript Accepted: 12 MAY 2008
- Manuscript Revised: 9 MAY 2008
- Manuscript Received: 14 MAR 2008
- NIH. Grant Numbers: CA113472, GM071424
- University of Colorado Cancer Center
- Canadian Institutes of Health Research
- National Cancer Institute of Canada
- PHD domain;
- tumor suppressors
Chromatin dynamics regulates diverse nuclear processes and influences cellular viability and tumorigenesis. The discrete chromatin states are linked to covalent histone modifications that control the extent of DNA accessibility to transacting factors. One of the most common epigenetic modifications is methylation of histone H3 at lysine 4 (H3K4). Lys4 can be mono-, di-, or tri-methylated, and the tri-methylated mark (H3K4me3) is normally associated with euchromatin and active gene transcription.1, 2
The inhibitor of growth (ING) family of tumor suppressors contain a C-terminal plant homeodomain (PHD) finger. This conserved zinc-binding module is found in many nuclear proteins including transcription factors, histone modifying enzymes, and ATP dependent chromatin remodeling complexes.3–5 A subset of PHD fingers has recently been shown to bind methylated and unmodified histone tails,5–9 with the H3K4me3 mark being specifically recognized by ING proteins. Unlike ING1 and ING2, which have been identified as components of histone deacetylase (HDAC) complexes, ING5 associates with histone acetyltransferase (HAT) complexes containing MOZ (monocytic leukemia zinc finger protein)/MORF (MOZ-related factor) and HBO1.10
To establish the structural basis of chromatin targeting by the HAT associated ING proteins, we determined the crystal structure of the ING5 PHD finger in complex with its histone target (H3K4me3). We also measured binding affinities for unmodified, mono-, di-, and tri-methylated histone peptides, and showed that both full-length ING5 and methylated H3K4 are essential for the acetyltransferase activity of the MOZ/MORF and HBO1 complexes. This functional data are the first direct evidence supporting the critical role of ING5 in directing the MOZ/MORF and HBO1 complexes to chromatin, which consequently increases the local HAT activity and stimulates chromatin remodeling.
Protein expression and purification
To produce ING5 for structural studies, the PHD domain (residues 178–236) was subcloned into a pGEX-2T vector (Amersham). The ING5 PHD finger was expressed in E. coli and purified essentially as described in Ref. 7. To assemble the MORF complexes, baculovirus expressing the HAT component as a Flag-tagged fusion protein was used to infect Sf9 cells, along with baculoviruses expressing HA-tagged BRPF1 and ING5. Extracts were affinity purified on M2 agarose (Sigma-Aldrich) as described.11 Generation of the baculovirus for the HAT component of MORF12 was reported previously,11 whereas baculovirus for HA-BRPF1 and HA-ING5 will be described elsewhere (Mukta Ullah, Jacques Côté & Xiang-Jiao Yang, unpublished data).
The ING5 PHD finger was crystallized in complex with the H3K4me3 histone tail peptide essentially as described in Ref. 7. All peptides were synthesized at the University of Colorado at Denver peptide core facility and contain the N-terminus of human histone H3, spanning amino acids 1 to 12 (ARTKQTARKSTG). These peptides were either unmodified, mono-, di-, or tri-methylated at K4. Crystals of the ING5-H3K4me3 peptide complex grew at 18°C in 1.6M sodium citrate at pH 6.5. All crystals grew to ∼0.05 × 0.05 × 0.3 mm3 in a tetragonal space group (P43) with unit cell parameters of a = 68.16 Å, b = 68.16 Å, c = 27.96 Å, α = β = γ = 90°. Crystals were flash cooled in liquid nitrogen, and X-ray data were collected at 100 K on a “NOIR-1” MBC system detector at beam line 4.2.2 at the Advanced Light Source in Berkeley, CA. A complete Zn2+ MAD data set to 1.75 Å was collected for peak, inflection, and remote wavelengths. Data were processed with D*TREK13 and statistics are shown in Table I. The phase problem was solved and the initial model built with SOLVE/RESOLVE.14, 15 The protein structure was further refined with CNS16 and O,17 and verified with PROCHECK.18
|Diffraction data collection|
|Unit cell parameters||a = b = 68.14, c = 28.25 Å, α = β = γ = 90°|
|Two molecules per asymmetric unit|
|Redundancya||6.95 (5.23)||6.84 (4.71)||6.82 (4.69)|
|Completeness (%)||98.3 (84.8)||96.9 (77.2)||96.9 (77.2)|
|Rmergeb||0.070 (0.219)||0.075 (0.213)||0.074 (0.249)|
|I/σ(I)||17.7 (2.8)||16.8 (2.7)||15.9 (2.5)|
|Refinement statistics (|F| > 0)|
|Resolution range (Å)||34–1.75|
|Number of protein atoms||979|
|Number of nonprotein atoms||144 water molecules and 4 zinc ions|
|R.m.s.d. from ideal bond length (Å)||0.007|
|R.m.s.d. from ideal bond angle (°)||1.336|
|Average B factor (Å2)||20.38|
|Most favored (%)||89.7|
|Additionally allowed (%)||6.2|
|Generously allowed (%)||2.1|
Tryptophan fluorescence spectra were recorded at 25°C on a Fluoromax3 spectrofluorimeter. The samples of 10 μM ING5 PHD finger containing progressively increasing concentrations of histone H3 peptides (up to 1 mM) were excited at 295 nm. Emission spectra were recorded between 305 and 405 nm with a 0.5 nm step size and a 1 s integration time, and averaged over 3 scans. The KDs were determined by fitting to the quadratic function using Eq. (1), where Fi is the fluorescence change, Fs is the fluorescence change at saturation of XT (the total protein concentration) and YT is the peptide concentration. The KD value is the average of three independent experiments.
Histone acetyltransferase assay
HAT assays were performed using histone peptides as previously described.19 Briefly, recombinant protein complexes affinity purified from insect cells (baculovirus) were incubated without or with 300 ng of biotinylated peptides corresponding to amino acids 1–21 of human histone H3 followed by a GG linker and biotinylated lysine (ARTKQTARKSTGGKAPRKQLA-GGK-biotin) (Upstate/Millipore) in 15 μL final volume of 50 mM KCl, 50 mM Tris (pH 8), 1 mM DTT, 5% glycerol, 10 mM Na-Butyrate, and 0.1 mM EDTA with 0.5 μL of [3H] acetyl-CoA (0.25 μCi/μL, 4.9 Ci/mmol) for 30 min at 30°C. Each reaction was spotted onto p81 filters, which were then washed three times with 50 mM Na Carbonate (pH 9.2). The amount of incorporated [3H] acetyl was determined using a scintillation counter. The standard deviation was calculated from duplicate assays of 2 independent experiments (4 values).
RESULTS AND DISCUSSION
Overall structure and monomer architecture
The PHD domain of human ING5 in complex with the H3K4me3 peptide crystallized in space group P43 with two complexes in the crystallographic asymmetric unit. Chains A and C represent the ING5 molecules, while chains B and D designate the H3K4me3 histone peptides. The crystal structure was solved by the Zn-MAD method at 1.75 Å resolution and was refined to an Rwork of 18.65% and Rfree of 19.86%. Diffraction data and refinement statistics are given in Table I. The two monomers in the asymmetric unit are in identical conformations and are superimposable with an rmsd of 0.34 Å over 51 pairs of Cα atoms.20 The overall architecture of the ING5 PHD finger exhibits structural features characteristic of other PHD modules and contains two zinc-binding clusters. The first zinc ion (Zn1) is coordinated by one histidine and three cysteine residues, while the other zinc ion (Zn2) is coordinated by four cysteine residues. The zinc ions stabilize three loops: L1 (residues 194–197), L2 (residues 202–210) and L3 (residues 218–229) [Fig. 1(A)]. A double stranded antiparallel beta sheet forms the core of the domain, and two short beta strands are seen at the N-terminus. The β3 and β4 strands contain residues 197–202 and 210–214, respectively, whereas two short alpha helices span residues 215–217 and 230–233. In the crystal structure, residues 178–184 and 236 are disordered in chain A of the ING5 PHD domain, and there is no density for residues 178–183 and 236 in chain C.
Coordination of the H3K4me3 peptide
The H3K4me3 peptide binds to the ING5 PHD domain via formation of a third antiparallel beta strand that pairs with the protein's central β3-β4 beta sheet [Fig. 1(A)]. These beta strand contacts include the peptides Arg2, Lys4, and Thr6 hydrogen bonding with G201, M199, and E197 residues of the PHD domain. Additional backbone contacts are formed between the amino group of Ala1 and the carbonyl groups of G225 and P223. The side chain hydroxyl moiety of S195 makes a hydrogen bond with Thr6, whereas the guanidino group of Arg2 interacts with D203 and C202 [Fig. 1(B)]. The H3K4me3 peptide lies in an extended groove of the ING5 PHD domain with tri-methylated Lys4 fitting snugly into a hydrophobic pocket [Fig. 1(C)]. The three sides of the hydrophobic pocket are formed by W211, S195, and Y188. Y188 is positioned near the tip of the me3 moiety, whereas M199 makes up the bottom of the pocket.
ING5 is a component of the MOZ/MORF HAT complex
ING5 was previously shown to copurify with the MOZ/MORF and HBO1 HAT complexes which acetylate histone H3 at lysine 14, and histone H4 at lysine 5, 8, and 12, respectively.10 However, the function of ING5 within these complexes remains unknown. To determine the role of ING5 in the MOZ/MORF and HBO1 HATs, recombinant complexes were purified from baculovirus and tested in HAT assays [Fig. 1(E)]. MORF HAT activity was first measured without histone peptides, and then in the presence of unmodified H3 (residues 1–21), H3K4me2, and H3K9me2 peptides. As shown in Figure 1(E), the MORF HAT alone exhibits low enzymatic activity. When the BRPF1 subunit of the MORF HAT complex was added, the HAT activity increased in the presence of all histone peptides; however, addition of the ING5 subunit caused MORF to preferentially acetylate the H3K4me2 peptide. The presence of ING5 was also necessary for acetylation of the H3K4me2 peptide by native HBO1 complexes (Saksouk and Côté, unpublished data). Thus, our data indicate that specific association of the ING5 PHD finger with methylated H3K4 is required for the robust HAT activity of both MORF and HBO1 complexes.
Structural comparison with other PHD domains
While the overall fold of the ING5 PHD finger is comparable with that of the ING2 PHD domain (PDB 2G6Q), ING5 has two additional elements, the N-terminal β-sheet and an α-helix. These structural variations, and a 20% difference in the primary sequence, account for the modest (1.06 Å) rmsd between the two structures. A structural comparison of the ING5 PHD domain with the PHD fingers of RAG2 (PDB 2v89), BPTF (2fuu), and Pygo1 (2dx8) reveals that it is most closely related to the Pygo1 PHD domain (rmsd of 1.82 Å) [Fig. 1(D)]. This is followed by BPTF (rmsd of 1.97 Å) and RAG2 (rmsd of 2.86 Å), respectively. Unlike ING5, the PHD fingers of RAG2, Pygo1, and BPTF all contain an alpha helix in loop 3. Pygo1 forms a dimer via this helix and an adjacent β-strand, which is not found in the other PHD domains. Additionally, a large insertion in loop 1 is a unique feature of RAG2.
The general histone-binding mode is conserved among the majority of PHD fingers. Much like the ING2 and BPTF PHD modules, the ING5 PHD finger binds H3K4me3 with a low μM affinity and selects for tri-methylated, over di-, mono-, or unmodified peptides (Table II). The major difference appears to be in coordination of K4me3, which is caged by four aromatic residues in BPTF, but by only two in ING5. In contrast, the RAG2 PHD finger prefers the H3R2me2/K4me2s double modification.24 This is most likely because of the replacement of G201 in ING5 with a tyrosine in RAG2, which prevents proper coordination of unmodified Arg2.24 It is notable that the BPTF and RAG2 PHD fingers share only 25% and 17% sequence identity with the ING5 PHD domain, respectively; yet they possess a highly conserved three-dimensional structure. This provides a good example of convergent evolution of dissimilar and unrelated proteins driven by the common function of histone binding.
|Histone peptide||KD ± s.d. (μM)|
|H3K4me0||261 ± 34|
|H3K4me1||222 ± 17|
|H3K4me2||16 ± 1.2|
|H3K4me3||2.4 ± 1.0|
Our results demonstrate that the PHD domain of ING5 has the characteristic tertiary fold of a zinc finger. It specifically recognizes the H3K4me3 histone peptide by forming an extended binding groove. Within this groove, a hydrophobic pocket clasps tri-methylated Lys4, discriminating against histone peptides containing di-, mono-, and unmodified Lys4. Arg2 of the peptide is coordinated in an adjacent pocket. The two pockets are connected by a narrow channel, which requires a small residue at position 3 of the histone tail. Together these features define the specificity of the ING5 PHD finger toward H3K4me3.
ING5 is a native subunit of the MOZ/MORF and HBO1 complexes, and is necessary for their enzymatic activity.10 Our data indicate that the presence of full-length ING5 results in preferential acetylation of histone peptides with the methylated H3K4 modification. These results suggest that ING5 acts as an adapter molecule which bridges and/or stabilizes the MOZ/MORF and HBO1 HAT complexes at the chromatin through binding to methylated H3K4 via its PHD finger. The structural and functional characterization of the ING5 tumor suppressor presented here is essential step in providing a fundamental link between HAT activity and chromatin remodeling. Understanding the biochemical mechanisms by which ING5 regulates cellular growth, proliferation, and stress responses are critical for establishing the significance of its altered function in normal and cancerous cells.
We thank Jay Nix at beam line 4.2.2 of the ALS in Berkeley, CA, for help with data collection. J.C. holds a Canada Research Chair. K.S.C. is supported by an American Cancer Society Postdoctoral Fellowship, and P.V.P. is a recipient of the NIH NRSA Predoctoral Fellowship.
- 22The PyMOL molecular graphics system. Palo Alto, CA: DeLano Scientific; 2002..