Salvianolic acid B inhibits the amyloid formation of human islet amyloid polypeptideand protects pancreatic beta-cells against cytotoxicity

Authors

  • Biao Cheng,

    1. Department of Bio-pharmaceutics, Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China
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  • Hao Gong,

    1. Department of Bio-pharmaceutics, Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China
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  • Xiaochao Li,

    1. Department of Bio-pharmaceutics, Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China
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  • Yue Sun,

    1. Department of Cell Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei, 430072, People's Republic of China
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  • Hong Chen,

    1. Department of Bio-pharmaceutics, Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China
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  • Xin Zhang,

    1. Department of Bio-pharmaceutics, Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China
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  • Qian Wu,

    1. Department of Bio-pharmaceutics, Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China
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  • Ling Zheng,

    Corresponding author
    1. Department of Cell Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei, 430072, People's Republic of China
    • College of Life Sciences, Wuhan University, Wuhan, China 430072
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  • Kun Huang

    Corresponding author
    1. Department of Bio-pharmaceutics, Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China
    2. Centre for Biomedicine Research, Wuhan Institute of Biotechnology, Wuhan, Hubei, 430070, People's Republic of China
    • Tongji School of Pharmacy, Huazhong University of Sci and Tech, Wuhan, China, 430030
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Abstract

The misfolding of human islet amyloid polypeptide (hIAPP) is regarded as one of the causative factors of type 2 diabetes mellitus (T2DM). Salvia miltiorrhiza (Danshen), one of the most commonly used of traditional Chinese medicines, is often used in Compound Recipes for treating diabetes, however with unclear mechanisms. Since salvianolic acid B (SalB) is the most abundant bioactive ingredient of salvia miltiorrhiza water-extract. In this study, we tested whether SalB has any effect on the amyloidogenicity of hIAPP. Our results clearly suggest that SalB can significantly inhibit the formation of hIAPP amyloid and disaggregate hIAPP fibrils. Furthermore, photo-crosslinking based oligomerization studies suggest SalB significantly suppresses the toxic oligomerization of hIAPP monomers. Cytotoxicity protection effects on pancreatic INS-1 cells by SalB were also observed using MTT-based assays, potentially due to the inhibition on the membrane disruption effects and attenuated mitochondria impairment induced by hIAPP. These results provide evidence that SalB may further be studied on the possible pharmacological treatment for T2DM. Proteins 2013. © 2012 Wiley Periodicals, Inc.

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