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Crystal structure of JHP933 from Helicobacter pylori J99 shows two-domain architecture with a DUF1814 family nucleotidyltransferase domain and a helical bundle domain

Authors

  • Ji Young Yoon,

    1. Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea
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    • Ji Young Yoon and Sang Jae Lee contributed equally to this study.

  • Sang Jae Lee,

    1. The Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul, Republic of Korea
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    • Ji Young Yoon and Sang Jae Lee contributed equally to this study.

  • Do Jin Kim,

    1. Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea
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  • Bong-Jin Lee,

    1. The Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul, Republic of Korea
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  • Jin Kuk Yang,

    Corresponding author
    1. Department of Chemistry, College of Natural Sciences, Soongsil University, Seoul, Republic of Korea
    • Correspondence to: Jin Kuk Yang; Department of Chemistry, College of Natural Sciences, Soongsil University, Seoul 156-743, Korea. E-mail: jinkukyang@ssu.ac.kr or Se Won Suh, Department of Chemistry; College of Natural Sciences, Seoul National University, Seoul 151-747, Korea. E-mail: sewonsuh@snu.ac.kr

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  • Se Won Suh

    Corresponding author
    1. Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea
    2. Department of Biophysics and Chemical Biology, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea
    • Correspondence to: Jin Kuk Yang; Department of Chemistry, College of Natural Sciences, Soongsil University, Seoul 156-743, Korea. E-mail: jinkukyang@ssu.ac.kr or Se Won Suh, Department of Chemistry; College of Natural Sciences, Seoul National University, Seoul 151-747, Korea. E-mail: sewonsuh@snu.ac.kr

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ABSTRACT

The jhp0933 gene in the plasticity region of Helicobacter pylori J99 encodes a hypothetical protein (JHP933), which may play some roles in pathogenesis. Here, we have determined the crystal structure of JHP933 at 2.17 Å. It represents the first crystal structure of the DUF1814 protein family. JHP933 consists of two domains: an N-terminal domain of the nucleotidyltransferase (NTase) fold and a C-terminal helix bundle domain. A highly positively charged surface patch exists adjacent to the putative NTP binding site. Structural similarity of JHP933 to known NTases is very remote, suggesting that it may function as a novel NTase. Proteins 2014; 82:2275–2281. © 2014 Wiley Periodicals, Inc.

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