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prp258-sup-0001-FigS1.tiffTIFF image1589K

Figure S1. Double immunofluorescence staining of mice lungs. Duoplex immunofluorescence staining of luciferase/CK18 (epithelial cell marker) or luciferase/CD31(endothelial cell marker) were performed on paraformaldehyde-fixed lung sections, as described in Materials and Methods. Double staining of lung obtained from saline-challenged NF- κB-luc mice showed normal staining of epithelial cells (A) and endothelial cells (C) for CK18 and CD31, respectively. On the contrary, no staining for luciferase was observed (B and D). Original magnification: 210×.

Figure S2. (A–E). LPS-induced cytokines in bronchoalveolar lavage fluid (BALF): effect of azithromycin. Transient NF-κB-luc transgenic mice were pretreated with saline or azithromycin (AZI, 100–600 mg/kg, per os) 4 h prior to LPS and sacrificed 24 h after tracheal instillation of LPS. A: IL1-α. B: IL1-b. C: IL-12. D: IL-12(p70). E: KC. Values are shown as mean ± SEM, n = 6 for each group.

prp258-sup-0002-FigS2.tiffTIFF image588K

Figure S2. (A–E). LPS-induced cytokines in bronchoalveolar lavage fluid (BALF): effect of azithromycin. Transient NF-κB-luc transgenic mice were pretreated with saline or azithromycin (AZI, 100–600 mg/kg, per os) 4 h prior to LPS and sacrificed 24 h after tracheal instillation of LPS. A: IL1-α. B: IL1-b. C: IL-12. D: IL-12(p70). E: KC. Values are shown as mean ± SEM, n = 6 for each group.

prp258-sup-0003-TableS1.docxWord document20K

Table S1. Cytokines analyred in BAL of LPS-challenged mice. Cytokines modulated by LPS showed a statistically significant increase in BAL concentrations obtained after LPS challenge.

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