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Isolation and antifungal activity of kakuol, a propiophenone derivative from Asarum sieboldii rhizome

Authors

  • Jung Yeop Lee,

    1. Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul 136-713, Korea
    Current affiliation:
    1. Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109-2216, USA
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  • Surk Sik Moon,

    1. Department of Chemistry, Kongju National University, Kongju 314-701, Korea
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  • Byung Kook Hwang

    Corresponding author
    1. Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul 136-713, Korea
    • Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul 136-701, Korea
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Abstract

An antifungal substance active against Colletotrichum orbiculare (Berk & Mont) Arx was isolated from the methanol extracts of Asarum sieboldii (Miq) Maek rhizomes. High-resolution MS, NMR and UV spectral data confirmed that the antifungal substance is kakuol, 2-hydroxy-4,5-methylenedioxypropiophenone. Colletotrichum orbiculare was most sensitive to kakuol, with MIC of 10 µg ml−1. Kakuol also completely inhibited the mycelial growth of Botrytis cinerea Pers ex Fr and Cladosporium cucumerinum Ellis & Arthur at 50 µg ml−1 and 30 µg ml−1, respectively. However, no antimicrobial activity was found against yeast and bacteria even at 100 µg ml−1. Kakuol exhibited a protective activity against the development of anthracnose disease on cucumber plants. The control efficacy of kakuol against the anthracnose disease was in general somewhat less than that of the commercial fungicide chlorothalonil. This is the first report to demonstrate in vitro and in vivo antifungal activity of kakuol against C orbiculare infection. Copyright © 2005 Society of Chemical Industry

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