A novel assay reveals the blockade of esterases by piperonyl butoxide

Authors


Abstract

BACKGROUND: Conventional in vitro assays sometimes fail to reveal esterase inhibition by piperonyl butoxide (PBO), although synergism studies suggest loss of esterase-mediated sequestration of insecticide does take place. A new in vitro assay has been devised that routinely reveals binding between PBO and these esterases.

RESULTS: The new ‘esterase interference’ assay detects the blockade of resistance-associated esterases in a species, Myzus persicae Sülzer, where this has not previously been seen. The assay also demonstrates directly the protective effect esterases may confer to target sites of insecticides.

CONCLUSION: The new assay reveals esterase blockade by PBO and thus has the potential to be used as a high-throughput screening method for other potential synergists. Copyright © 2008 Society of Chemical Industry

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