Biological effects of rice harbouring Bph14 and Bph15 on brown planthopper, Nilaparvata lugens

Authors

  • Jie Li,

    1. Hubei Insect Resources Utilisation and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
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  • Qiuhong Chen,

    1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
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  • Liangquan Wang,

    1. Hubei Insect Resources Utilisation and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
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  • Jia Liu,

    1. Hubei Insect Resources Utilisation and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
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  • Keke Shang,

    1. Hubei Insect Resources Utilisation and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
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  • Hongxia Hua

    Corresponding author
    1. Hubei Insect Resources Utilisation and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
    • College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
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Abstract

BACKGROUND: Brown planthopper (Nilaparvata lugens Stål; BPH) resistance genes Bph14 and Bph15 have been introgressed singly or pyramided into rice variety Minghui 63 (MH63). The antibiosis and antixenosis effects of these rice lines on BPH and the expression of five P450 genes of BPH regulated by these rice lines were investigated in this study.

RESULTS: The resistance level of rice lines harbouring resistance genes was improved compared with MH63. MH63::14 (carrying Bph14) had negative effects on the development of males, honeydew excretion of females, the female ratio and the copulation rate compared with MH63. MH63::14 also exhibited antixenosis action against BPH nymphs, female adults and oviposition. Besides these negative effects, MH63::15 (carrying Bph15) could also retard the development of females, lower the fecundity and shorten the lifespan of females. The antixenosis action of MH63::15 was stronger than that of MH63::14. When Bph14 and Bph15 were pyramided, antibiosis and antixenosis effects were significantly enhanced relative to single-introgression lines. Among the five P450 genes of BPH, expression of three genes was upregulated, one gene was downregulated and one gene was unchanged by resistant hosts.

CONCLUSION: Both Bph14 and Bph15 could improve resistance levels of MH63. MH63::15 and MH63::14&15 had greater potential to control BPH infestations than MH63::14. Copyright © 2011 Society of Chemical Industry

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