Get access

Identification and expression profiles of nine glutathione S-transferase genes from the important rice phloem sap-sucker and virus vector Laodelphax striatellus (Fallén) (Hemiptera: Delphacidae)

Authors

  • Wen-Wu Zhou,

    1. State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    Search for more papers by this author
  • Xi-Wang Li,

    1. State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    Search for more papers by this author
  • Yin-Hua Quan,

    1. Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China
    Search for more papers by this author
  • Jiaan Cheng,

    1. State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    Search for more papers by this author
  • Chuan-Xi Zhang,

    1. State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    Search for more papers by this author
  • Geoff Gurr,

    1. State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    2. EH Graham Centre for Agricultural Innovation, Charles Sturt University, Orange, Australia
    Search for more papers by this author
  • Zeng-Rong Zhu

    Corresponding author
    1. State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    • State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
    Search for more papers by this author

Abstract

BACKGROUND: Glutathione S-transferases (GSTs) have received considerable attention in insects for their roles in insecticide resistance. Laodelphax striatellus (Fallén) is a serious rice pest. L. striatellus outbreaks occur frequently throughout eastern Asia. A key problem in controlling this pest is its rapid adaptation to numerous insecticides. In this research, nine cDNAs encoding GSTs in L. striatellus were cloned and characterised.

RESULTS: The cloned GSTs of L. striatellus belonged to six cytosolic classes and a microsomal subgroup. Exposure to sublethal concentrations of each of the six insecticides, DDT, chlorpyrifos, fipronil, imidacloprid, buprofezin and beta-cypermethrin, quickly induced (6 h) up-expression of LsGSTe1. The expression of LsGSTs2 was increased by chlorpyrifos, fipronil and beta-cypermethrin. Furthermore, exposure of L. striatellus to fipronil, imidacloprid, buprofezin and beta-cypermethrin increased the expression of the LsGSTm gene after 24 or 48 h.

CONCLUSION: This work is the first identification of GST genes from different GST groups in Auchenorrhyncha species and their induction characteristics with insecticide types and time. The elevated expression of GST genes induced by insecticides might be related to the enhanced tolerance of this insect to insecticides and xenobiotics. Copyright © 2012 Society of Chemical Industry

Ancillary