Abdominal color of the Asian citrus psyllid, Diaphorina citri, is associated with susceptibility to various insecticides
Article first published online: 28 NOV 2012
© 2012 Society of Chemical Industry
Pest Management Science
Volume 69, Issue 4, pages 535–541, April 2013
How to Cite
Tiwari, S., Killiny, N., Mann, R. S., Wenninger, E. J. and Stelinski, L. L. (2013), Abdominal color of the Asian citrus psyllid, Diaphorina citri, is associated with susceptibility to various insecticides. Pest. Manag. Sci., 69: 535–541. doi: 10.1002/ps.3407
- Issue published online: 20 MAR 2013
- Article first published online: 28 NOV 2012
- Accepted manuscript online: 21 AUG 2012 12:00AM EST
- Manuscript Accepted: 21 AUG 2012
- Manuscript Revised: 8 AUG 2012
- Manuscript Received: 16 MAY 2012
- CYP4 genes;
- cytochrome P450 monooxygenase;
- gene expression;
- citrus greening
Color morphs of an insect species are known to vary in activities of detoxifying enzymes and associated susceptibility to insecticides. In Diaphorina citri, three color morphs are known to occur. In the present study, susceptibility to four insecticides was compared among gray/brown, blue/green and orange/yellow color morphs of field-collected D. citri.
The orange/yellow morph was significantly more susceptible to fenpropathrin than the blue/green morph, and imidacloprid and chlorpyrifos caused higher mortality in the orange/yellow morph than in the blue/green and gray/brown morphs. To confirm the genetic basis of variable levels of susceptibility, the relative expression of five CYP4 genes was compared among the color morphs. CYP4C67 was expressed at significantly higher levels in the blue/green than in the orange/yellow and gray/brown morphs. CYP4DA1, CYP4C68, CYP4G70 and CYP4DB1 were expressed at significantly higher levels in the blue/green and gray/brown morphs than in the orange/yellow morph. Lower expression of CYP4 genes in the orange/yellow morph as compared with the others was correlated with reduced signal of 45 kD cytochrome P450 proteins, as determined by the western blot.
The results indicate differential susceptibility of D. citri color morphs to insecticides, which will need to be accounted for in future insecticide monitoring programs and may affect management programs.