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ALS herbicide resistance mutations in Raphanus raphanistrum: evaluation of pleiotropic effects on vegetative growth and ALS activity

Authors

  • Mei Li,

    1. Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, WA, Australia
    2. Institute of Plant Protection, Shandong Academy of Agricultural Sciences, JiNan, China
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  • Qin Yu,

    1. Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, WA, Australia
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  • Heping Han,

    1. Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, WA, Australia
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  • Martin Vila-Aiub,

    Corresponding author
    1. IFEVA-CONICET – Facultad de Agronomía, Universidad de Buenos Aires (UBA), Argentina
    • Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, WA, Australia
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  • Stephen B Powles

    1. Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, WA, Australia
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Correspondence to: Martin Vila-Aiub, Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, WA 6009, Australia. E-mail: vila@ifeva.edu.ar

Abstract

Background

Gene mutations that endow herbicide resistance may cause pleiotropic effects on plant ecology and physiology. This paper reports on the effect of a number of known and novel target-site resistance mutations of the ALS gene (Ala-122-Tyr, Pro-197-Ser, Asp-376-Glu or Trp-574-Leu) on vegetative growth traits of the weed Raphanus raphanistrum.

Results

The results from a series of experiments have indicated that none of these ALS resistance mutations imposes negative pleiotropic effects on relative growth rate (RGR), photosynthesis and resource-competitive ability in R. raphanistrum plants. The absence of pleiotropic effects on plant growth occurs in spite of increased (Ala-122-Tyr, Pro-197-Ser, Asp-376-Glu) and decreased (Trp-574-Leu) extractable ALS activity.

Conclusion

The absence of detrimental pleiotropic effects on plant growth associated with the ALS target-site resistance mutations reported here is a contributing factor in resistance alleles being at relatively high frequencies in ALS-herbicide-unselected R. raphanistrum populations.© 2012 Society of Chemical Industry

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