Effects of Cry34/35Ab1 corn on the survival and development of western corn rootworm, Diabrotica virgifera virgifera


  • Given, in part, on 13 December 13 2010 at the 58th Annual Meeting of the Entomological Society of America in San Diego, California.

Correspondence to: Melissa L Rudeen, Ecology, Evolution and Behavior Department, University of Minnesota, 1987 Upper Buford Circle, 100 Ecology Building, St Paul, MN 55108, USA. E-mail: ryne0008@umn.edu



The western corn rootworm, Diabrotica virgifera virgifera LeConte, is a major agricultural pest that is managed with transgenic corn, Zea mays L., expressing genes from Bacillus thuringiensis Berliner (Bt). The effects of Bt corn producing Cry34/35Ab1 (event DAS-59122–7) and entomopathogens on the survival and development of larval D. v. virgifera were examined in laboratory, field and greenhouse experiments.


Larvae preferred non-Bt over Bt corn in a laboratory experiment, and there was higher recovery from non-Bt corn than from Bt corn in a field experiment. In a greenhouse study, survival at 17 days did not differ significantly among non-Bt corn, Bt corn and a blend of Bt and non-Bt corn, but development was delayed on Bt corn. Older larvae fed non-Bt corn had lower survival when entomopathogenic nematodes were added, but no other effects of pathogen were detected.


Bt corn producing Cry34/35Ab1 delayed larval development of D. v. virgifera and deterred feeding. In a mixture of Bt and non-Bt corn, larval development and survival were similar to non-Bt corn alone, suggesting that non-Bt plants in a blended refuge or a pure stand may produce a similar number of adult insects, and that timing of adult emergence may also be similar. © 2012 Society of Chemical Industry