Antagonistic bacterium Bacillus amyloliquefaciens induces resistance and controls the bacterial wilt of tomato

Authors


Correspondence to: Yangchun Xu, Jiangsu Provincial Key Lab of Organic Solid Waste Utilisation, Nanjing Agricultural University, Nanjing 210095, China. E-mail: ycxu@njau.edu.cn

Abstract

Background

Bacterial wilt caused by Ralstonia solanacearum (RS) is a serious threat for agricultural production. In this study, Bacillus amyloliquefaciens strains CM-2 and T-5 antagonistic to RS were used to create bioorganic fertilisers to control tomato wilt under greenhouse conditions. The possible mechanism of resistance inducement by the antagonistic bacteria was also evaluated.

Results

The application of bioorganic fertilisers significantly reduced incidences of tomato wilt (by 63–74%), promoted plant growth and significantly reduced the RS populations in rhizosphere compared with the control. Both strains CM-2 and T-5 applied with bioorganic fertilisers survived well in the tomato rhizosphere. Tomato seedlings treated with cell suspension of T-5 followed by challenge inoculation with RS increased the activities of polyphenol oxidase, phenylalanine ammonia lyase and peroxidase compared with the untreated control. Furthermore, the expressions of the marker genes responsible for synthesis of phytohormones salicylic acid, ethylene and jasmonic acid in seedlings treated with T-5 in response to inoculated pathogen were significantly higher.

Conclusions

This study suggests that strains CM-2 and T-5 containing bioorganic fertilisers effectively control tomato wilt. Increased enzyme activities and expression of defence genes in plants indicated that the antagonistic bacteria induced plant resistance, which was the potential biocontrol mechanism of tomato wilt. © 2013 Society of Chemical Industry

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