Susceptibility status of Culex quinquefasciatus (Diptera: Culicidae) populations to the chemical insecticide temephos in Pernambuco, Brazil

Authors

  • Liliane Barbosa Amorim,

    1. Department of Entomology, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife-PE, Brazil
    2. Postgraduate Programme of Animal Biology/UFPE
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  • Elisama Helvecio,

    1. Department of Entomology, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife-PE, Brazil
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  • Cláudia Maria Fontes de Oliveira,

    1. Department of Entomology, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife-PE, Brazil
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  • Constância Flávia Junqueira Ayres

    Corresponding author
    1. Department of Entomology, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife-PE, Brazil
    • Correspondence to: Constância Flávia Junqueira Ayres, Department of Entomology, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Av. Moraes Rego s/n, Cidade Universitária, Recife-PE 50670–420, Brazil. E-mail: tans@cpqam.fiocruz.br

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Abstract

BACKGROUND

Culex quinquefasciatus is the vector of many agents of human diseases, including Wuchereria bancrofti, the parasite that causes bancroftian filariasis, an endemic disease in Pernambuco State, Brazil. Although temephos is not currently used to control C. quinquefasciatus, the species might be under a selection process from incidental exposure to this compound. This study aimed to evaluate the susceptibility status of C. quinquefasciatus to temephos, using bioassays, and to investigate its putative resistance mechanisms through biochemical assays and screening of the G119S mutation in the acetylcholinesterase gene, which is associated with organophosphate resistance, carried out by PCR and sequencing.

RESULTS

The results showed that only mosquitoes from Santa Cruz do Capibaribe (SC) had an alteration in their susceptibility status (RR = 7.2-fold), while the other populations were all susceptible to the insecticide. Biochemical assays showed increased activity for all esterases in SC, as well as evidence of acetylcholinesterase insensitivity. The G119S mutation was detected in this population with a frequency of 0.11, but it was not found in the remaining populations.

CONCLUSION

These data show that mechanisms of temephos resistance have been selected in natural C. quinquefasciatus populations from Pernambuco, which could undermine future control actions. © 2013 Society of Chemical Industry

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