These authors contributed equally to this work.
Development of a DNA microarray for species identification of quarantine aphids
Article first published online: 10 MAY 2013
© 2013 Society of Chemical Industry
Pest Management Science
Volume 69, Issue 12, pages 1399–1406, December 2013
How to Cite
Lee, W. S., Choi, H., Kang, J., Kim, J.-H., Lee, S. H., Lee, S. and Hwang, S. Y. (2013), Development of a DNA microarray for species identification of quarantine aphids. Pest. Manag. Sci., 69: 1399–1406. doi: 10.1002/ps.3520
- Issue published online: 1 NOV 2013
- Article first published online: 10 MAY 2013
- Accepted manuscript online: 1 APR 2013 11:06AM EST
- Manuscript Accepted: 1 APR 2013
- Manuscript Revised: 8 FEB 2013
- Manuscript Received: 5 OCT 2012
- DNA microarray;
- species identification;
Aphid pests are being brought into Korea as a result of increased crop trading. Aphids exist on growth areas of plants, and thus plant growth is seriously affected by aphid pests. However, aphids are very small and have several sexual morphs and life stages, so it is difficult to identify species on the basis of morphological features. This problem was approached using DNA microarray technology.
DNA targets of the cytochrome c oxidase subunit I gene were generated with a fluorescent dye-labelled primer and were hybridised onto a DNA microarray consisting of specific probes. After analysing the signal intensity of the specific probes, the unique patterns from the DNA microarray, consisting of 47 species-specific probes, were obtained to identify 23 aphid species. To confirm the accuracy of the developed DNA microarray, ten individual blind samples were used in blind trials, and the identifications were completely consistent with the sequencing data of all individual blind samples.
A microarray has been developed to distinguish aphid species. DNA microarray technology provides a rapid, easy, cost-effective and accurate method for identifying aphid species for pest control management. © 2013 Society of Chemical Industry