Development of a DNA microarray for species identification of quarantine aphids

Authors

  • Won Sun Lee,

    1. Department of Biochemistry, Hanyang University and GenoCheck Co., Ltd, Ansan, Gyeonggi-do, Republic of Korea
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    • These authors contributed equally to this work.
  • Hwalran Choi,

    1. Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
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    • These authors contributed equally to this work.
  • JinSeok Kang,

    1. Department of Biochemistry, Hanyang University and GenoCheck Co., Ltd, Ansan, Gyeonggi-do, Republic of Korea
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  • Ji-Hoon Kim,

    1. Department of Biochemistry, Hanyang University and GenoCheck Co., Ltd, Ansan, Gyeonggi-do, Republic of Korea
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  • Si Hyeock Lee,

    1. Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
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  • Seunghwan Lee,

    Corresponding author
    1. Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
    • Correspondence to: Seung Yong Hwang, Department of Biochemistry, Hanyang University & GenoCheck Co., Ltd., Sangrok-gu, Ansan, Gyeonggi-do 426-791, Korea; and Seunghwan Lee, Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul 147–921, Republic of Korea. E-mails: syhwang@genocheck.com and seung@snu.ac.kr

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  • Seung Yong Hwang

    Corresponding author
    1. Department of Biochemistry, Hanyang University and GenoCheck Co., Ltd, Ansan, Gyeonggi-do, Republic of Korea
    • Correspondence to: Seung Yong Hwang, Department of Biochemistry, Hanyang University & GenoCheck Co., Ltd., Sangrok-gu, Ansan, Gyeonggi-do 426-791, Korea; and Seunghwan Lee, Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, Seoul 147–921, Republic of Korea. E-mails: syhwang@genocheck.com and seung@snu.ac.kr

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Abstract

BACKGROUND

Aphid pests are being brought into Korea as a result of increased crop trading. Aphids exist on growth areas of plants, and thus plant growth is seriously affected by aphid pests. However, aphids are very small and have several sexual morphs and life stages, so it is difficult to identify species on the basis of morphological features. This problem was approached using DNA microarray technology.

results

DNA targets of the cytochrome c oxidase subunit I gene were generated with a fluorescent dye-labelled primer and were hybridised onto a DNA microarray consisting of specific probes. After analysing the signal intensity of the specific probes, the unique patterns from the DNA microarray, consisting of 47 species-specific probes, were obtained to identify 23 aphid species. To confirm the accuracy of the developed DNA microarray, ten individual blind samples were used in blind trials, and the identifications were completely consistent with the sequencing data of all individual blind samples.

CONCLUSION

A microarray has been developed to distinguish aphid species. DNA microarray technology provides a rapid, easy, cost-effective and accurate method for identifying aphid species for pest control management. © 2013 Society of Chemical Industry

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