Improving the biocontrol potential of entomopathogenic nematodes against Mamestra brassicae: effect of spray application technique, adjuvants and an attractant

Authors

  • Bert Beck,

    Corresponding author
    1. Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Agricultural Engineering, Merelbeke, Belgium
    • Correspondence to: Bert Beck and David Nuyttens, Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Agricultural Engineering, Burgemeester van Gansberghelaan 115-1, 9820 Merelbeke, Belgium. E-mails: bert.beck@ilvo.vlaanderen.be and david.nuyttens@ilvo.vlaanderen.be

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  • Eva Brusselman,

    1. Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Agricultural Engineering, Merelbeke, Belgium
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  • David Nuyttens,

    Corresponding author
    1. Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Agricultural Engineering, Merelbeke, Belgium
    • Correspondence to: Bert Beck and David Nuyttens, Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Agricultural Engineering, Burgemeester van Gansberghelaan 115-1, 9820 Merelbeke, Belgium. E-mails: bert.beck@ilvo.vlaanderen.be and david.nuyttens@ilvo.vlaanderen.be

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  • Maurice Moens,

    1. Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Agricultural Engineering, Merelbeke, Belgium
    2. Department of Crop Protection, Ghent University, Ghent, Belgium
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  • Femke Temmerman,

    1. Inagro, Rumbeke-Beitem, Belgium
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  • Sabien Pollet,

    1. Inagro, Rumbeke-Beitem, Belgium
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  • Stephanie Van Weyenberg,

    1. Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Agricultural Engineering, Merelbeke, Belgium
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  • Pieter Spanoghe

    1. Department of Crop Protection, Ghent University, Ghent, Belgium
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Abstract

BACKGROUND

Steinernema carpocapsae Weiser, an entomopathogenic nematode (EPN), is a potential biological control agent for the cabbage moth (Mamestra brassicae L.). This research aimed to identify a suitable spray application technique, and to determine whether yeast extract added to an EPN spray has an attracting and/or a feeding stimulant effect on M. brassicae. The biological control capabilities of EPN against this pest were examined in the field.

RESULTS

Good coverage of the underside of cauliflower leaves, the habitat of young instar larvae (L1–L4) of M. brassicae was obtained using different spray boom configurations with vertical extensions that carried underleaf spraying nozzles. One of the configurations was selected for field testing with an EPN spray. Brewer's yeast extract stimulated larval feeding on leaves, and increased the mortality of these larvae when exposed to EPN. The field trial showed that a spray application with S. carpocapsae, Addit and xanthan gum can effectively lower the numbers of cabbage heads damaged by M. brassicae. Brewer's yeast extract did not significantly increase this field performance of EPN.

CONCLUSION

Steinernema carpocapsae, applied with an appropriate spray technique, can be used within biological control schemes as part of a resistance management programme for Bt. © 2013 Society of Chemical Industry

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