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ps3572-sup-0001-Table S1.docWord document37KTable S1 First PCR primers for a general cloning strategy
ps3572-sup-0002-Table S2.docWord document36KTable S2 Second PCR primers for nested Annealing Control Primer PCR
ps3572-sup-0003-Table S3.docWord document53KTable S3 Description of candidate reference genes and PCR primers
ps3572-sup-0004-Table S4.docWord document43KTable S4 Gene expression stability of reference candidate genes as predicted by NormFinder
ps3572-sup-0005-Table S5.docWord document82KTable S5 Primers used in real-time PCR
ps3572-sup-0006-Figure S1.docWord document1055KFig. S1 Dissociation curves for candidate reference genes. Dissociation curves for seven candidate reference genes derived from two technical replicates of 24 cDNA samples.
ps3572-sup-0007-Figure S2.docWord document34KFig. S2 Gene expression stability of reference candidate genes as predicted by geNormPLUS. A, Optimal number of reference genes. The optimal number of reference genes required for quantitative PCR data normalization was determined by the geNorm program. The pairwise variation (Vn/Vn+1) was analyzed between the normalization factors NFn and NFn+1. Cutoff V-value is 0.15. B, Stability of reference candidate genes. A lower M value indicates more stable expression. The stability of all the genes was lower than the cutoff M-value (1.5).

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