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Genetic toxicity of dillapiol and spinosad larvicides in somatic cells of Drosophila melanogaster

Authors

  • Eliezer H Pires Aciole,

    1. Programa de Pós-Graduação em Saúde Humana e Meio Ambiente (PPGSHMA), Universidade Federal de Pernambuco (UFPE), Centro Acadêmico de Vitória, Rua do Alto do Reservatório s/n, Bairro Bela Vista, CEP 55608-680, Vitória de Santo Antão, PE, Brasil
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  • Nilza N Guimarães,

    1. Universidade Federal de Goiás (UFG), Instituto de Ciências Biológicas, Campus II Bloco ICB 2 sala 103 Campus, CEP 74001-970, Caixa Postal 131, Laboratório de Genética Toxicológica, Goiânia, GO, Brasil
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  • Andre S Silva,

    1. Universidade Federal de Pernambuco (UFPE), Centro Acadêmico de Vitória, Rua do Alto do Reservatório s/n, Bairro Bela Vista, CEP 55608-680, Laboratório de Genética, Vitória de Santo Antão, PE, Brasil
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  • Erima M Amorim,

    1. Universidade Federal de Pernambuco (UFPE), Centro Acadêmico de Vitória, Rua do Alto do Reservatório s/n, Bairro Bela Vista, CEP 55608-680, Laboratório de Genética, Vitória de Santo Antão, PE, Brasil
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  • Sergio M Nunomura,

    1. Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pesquisa em Tecnologia e Inovação, Avenida André Araújo 2936, Bairro Aleixo, CEP 69083-000, Manaus, AM, Brasil
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  • Ana Cristina L Garcia,

    1. Universidade Federal de Pernambuco (UFPE), Centro Acadêmico de Vitória, Rua do Alto do Reservatório s/n, Bairro Bela Vista, CEP 55608-680, Laboratório de Genética, Vitória de Santo Antão, PE, Brasil
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  • Kênya S Cunha,

    1. Universidade Federal de Goiás (UFG), Instituto de Ciências Biológicas, Campus II Bloco ICB 2 sala 103 Campus, CEP 74001-970, Caixa Postal 131, Laboratório de Genética Toxicológica, Goiânia, GO, Brasil
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  • Claudia Rohde

    Corresponding author
    1. Universidade Federal de Pernambuco (UFPE), Centro Acadêmico de Vitória, Rua do Alto do Reservatório s/n, Bairro Bela Vista, CEP 55608-680, Laboratório de Genética, Vitória de Santo Antão, PE, Brasil
    • Correspondence to: Claudia Rohde, Universidade Federal de Pernambuco (UFPE), Centro Acadêmico de Vitória, Laboratório de Genética, Rua do Alto do Reservatório s/n, Bairro Bela Vista, CEP 55608-680, Vitória de Santo Antão, Pernambuco, Brazil E-mail: claudia.rohde@yahoo.com.br

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Abstract

BACKGROUND

Higher rates of diseases transmitted from insects to humans led to the increased use of organophosphate insecticides, proven to be harmful to human health and the environment. New, more effective chemical formulations with minimum genetic toxicity effects have become the object of intense research. These formulations include larvicides derived from plant extracts such as dillapiol, a phenylpropanoid extracted from Piper aduncum, and from microorganisms such as spinosad, formed by spinosyns A and D derived from the Saccharopolyspora spinosa fermentation process. This study investigated the genotoxicity of dillapiol and spinosad, characterising and quantifying mutation events and chromosomal and/or mitotic recombination using the somatic mutation and recombination test (SMART) in wings of Drosophila melanogaster.

RESULTS

Standard cross larvae (72 days old) were treated with different dillapiol and spinosad concentrations. Both compounds presented positive genetic toxicity, mainly as mitotic recombination events. Distilled water and doxorubicin were used as negative and positive controls respectively.

CONCLUSION

Spinosad was 14 times more genotoxic than dillapiol, and the effect was found to be purely recombinogenic. However, more studies on the potential risks of insecticides such as spinosad and dillapiol are necessary, based on other experimental models and methodologies, to ensure safe use. © 2013 Society of Chemical Industry

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