ps3645-sup-0001-FigureS1.docWord document407KFig. S1 Genotyping of Cyp6a2 alleles. (A) Allele specific PCR. Cyp6a2Canton-S-1 and Cyp6a2Canton-S-2 without the Nrf2/Maf binding site can be discriminated from other Cyp6a2 alleles with the intact Nrf2/Maf binding site. Primers used in each PCR were presented on the right of the gel picture. (B) PCR-RFLP. The PCR product of 91-C genotype can not be digested by Sau96I because “G1410” frameshift deletion disrupts the Sau96I restriction site.
ps3645-sup-0002-FigureS2.docWord document123KFig. S2 Sequence alignment of genomic Cyp6a2 alleles. Dots represent nucleotides deletions, and dashes represent identical nucleotides among these alleles. The position of nucleotide base was numbering with the translation start site as +1. The Nrf2/Maf binding site, frameshift mutation, the start codon and Stop codon and intron were boxed.
ps3645-sup-0003-TableS1.docWord document53KTable S1 Primers used in this study.

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