ps3657-sup-0001-FigureS1.docWord document1797KFigure S1 Expression intensity profiles of the 176 detoxification genes in the selected resistant strain JH-chl at G25 and the susceptible JHS at G25 based on semi-quantitative RT-PCR products. R, resistant strain; S, susceptible strain; P, cytochrome P450 monooxygenase; CE, carboxyesterase; PE, phosphoesterase; and GST, glutathione-S-transferase. The arrow next to the gel picture indicates upregulated expression of the detoxification gene in JH-chl sample. A, B, C and D denoted that comparisons of the 71 P450, 56 CE, 63 PE and 12 GST gene expression profiles in JH-chl vs. JHS, respectively. The β-actin was used as a reference control.
ps3657-sup-0002-FigureS2.docWord document6049KFigure S2 Full-length mRNA sequences of L. striatellus CYP6AY3v2, CYP306A2v2, CYP353D1v2 and LSCE36. The 5' and 3' untranslated regions are highlighted in black. The start codon and the stop codon are underlined in black. The predicted N-glycosylation sites are shown in blue. The signal peptide sequences are indicated by blue wavy underline. The residues of the catalytic triads are circled in red. The oxyanion hole residues are boxed in blue. Conserved domains common to cytochrome P450s and carboxylesterase are highlighted in yellow, which are the helix I motif, the helix K motif, the PERF motif, the heme-binding “signature” motif, carboxylesterase type-B signature 2 motif and carboxylesterase type-B serine motif. Marked in boxes are the terminal transmembrane anchor (black-line box) and the SRS 1–6 (red-line box).
ps3657-sup-0003-TableS1.docWord document34KTable S1 The primers used for quantitative real-time RT-PCR reaction.
ps3657-sup-0004-TableS2.docWord document41KTable S2 The primers used for RACE amplification.
ps3657-sup-0005-TableS3.docWord document38KTable S3 The primers used in RNAi experiments.

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