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Nitric-oxide-mediated cell death is triggered by chitosan in Fusarium eumartii spores

Authors

  • María Cecilia Terrile,

    1. Instituto de Investigaciones Biológicas, UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina
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    • Both of these authors contributed equally to this work.

  • Andrea Yamila Mansilla,

    1. Instituto de Investigaciones Biológicas, UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina
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    • Both of these authors contributed equally to this work.

  • Liliana Albertengo,

    1. Laboratorio de Investigaciones Básicas y Aplicadas de Quitina-Departamento de Química (INQUISUR), UE CONICET-UNS, Universidad Nacional del Sur, Bahía Blanca, Argentina
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  • María Susana Rodríguez,

    1. Laboratorio de Investigaciones Básicas y Aplicadas de Quitina-Departamento de Química (INQUISUR), UE CONICET-UNS, Universidad Nacional del Sur, Bahía Blanca, Argentina
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  • Claudia Anahí Casalongué

    Corresponding author
    1. Instituto de Investigaciones Biológicas, UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina
    • Correspondence to: Claudia A Casalongué, Instituto de Investigaciones Biológicas. UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina. E-mail: casalong@mdp.edu.ar

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Abstract

BACKGROUND

The genus Fusarium comprises a heterogeneous group of fungi important for agriculture. Fusarium solani f. sp. eumartii (F. eumartii), historically considered to be a fungal pathogen of potato, has also been associated with tomato disease. Currently, chitosan and its derivatives have been receiving more attention as environmentally friendly antimicrobial compounds in sustainable practices. The aim of the present work was to characterize downstream events associated with the mode of action of chitosan, including nitrosative reactive species, in order to identify new biomarkers of its cytotoxic action.

RESULTS

Data indicated that chitosan-mediated nitric oxide (NO) production might lead to conidial death, concomitant with the strong reduction in fungal pathogenicity in tomato plants. Following chitosan applications, a notably dose-dependent reduction in conidial viability was demonstrated in F. eumartii. Thereafter, the infectivity of chitosan-treated spores was tested by a bioassay using tomato seedlings.

CONCLUSION

All these data highlight NO valuable properties as a quantitative and qualitative biomarker of cytotoxic action of chitosan in conidial cells. In addition, these findings place the chitosan assayed here as a fungicide with a high potential of application in sustainable horticultural practices. © 2014 Society of Chemical Industry

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