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ps3814-sup-0001-FigureS1.tifTIFF image13407KChitosan induces H2O2 accumulation in F. eumartii spores. (A) Fungal spores were incubated with different concentrations of chitosan for 4 h and then stained with DAB. Uptake of DAB is confirmed by the dark staining (reddish-brown) reaction in the spores under light microscopy. Pictures show general phenomena representative for at least six individual experiments. Bar: 50 µM. (B) Percentage of DAB-stained spores as a function of chitosan concentrations. Different letters indicate statistically significant differences (Tukey test, P < 0.05). Error bars denote SD (n = 4).
ps3814-sup-0002-FigureS2.tifTIFF image7589KChitosan inhibits F. eumartii hyphal growth. (A) Mycelia of F. eumartii were grown in PDA media containing different concentrations of chitosan. Fungal growth was expressed as percentage of control treatment. Different letters indicate statistically significant differences (Tukey test, P < 0.05). Error bars denote SD (n = 3). (B) Representative pictures were taken after 3 days of fungal growth and shows general phenomena for at least three individual experiments.

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