Ying Huang and Li Ma contributed equally to this work.
Isolation and characterisation of rhizosphere bacteria active against Meloidogyne incognita, Phytophthora nicotianae and the root knot–black shank complex in tobacco
Article first published online: 28 MAY 2014
© 2014 Society of Chemical Industry
Pest Management Science
Volume 71, Issue 3, pages 415–422, March 2015
How to Cite
Huang, Y., Ma, L., Fang, D. H., Xi, J. Q., Zhu, M. L., Mo, M. H., Zhang, K. Q. and Ji, Y. P. (2015), Isolation and characterisation of rhizosphere bacteria active against Meloidogyne incognita, Phytophthora nicotianae and the root knot–black shank complex in tobacco. Pest. Manag. Sci., 71: 415–422. doi: 10.1002/ps.3820
- Issue published online: 10 FEB 2015
- Article first published online: 28 MAY 2014
- Accepted manuscript online: 2 MAY 2014 08:32AM EST
- Manuscript Accepted: 27 APR 2014
- Manuscript Revised: 25 APR 2014
- Manuscript Received: 24 JUL 2013
- disease complex;
- Meloidogyne incognita;
- Phytophthora nicotianae;
The use of dually antagonistic bacteria (DAB) as alternatives to chemicals for biological control of disease complexes has received little attention. In this study targeting the Meloidogyne incognita–Phytophthora nicotianae complex, DAB from the tobacco rhizosphere were identified and screened against the diseases caused by one or both pathogens in tobacco.
From 450 soil tobacco rhizosphere samples, 26 DAB were identified and had in vitro nematicidal and antifungal efficacies of 37.2–100% and 32.9–73.4% respectively. These DAB were classified into 19 species of 11 genera. In pot experiments, Streptomyces flavofungini SNA26, Pseudomonas putida SNB53 and Serratia marcescens subsp. sakuensis SNB54 effectively suppressed black shank (control effect 72.0–80.2%), root knot (70.0–81.7) and the disease complex (58.7–68.5%) caused by P. nicotianae, M. incognita and both pathogens in tobacco respectively.
Nineteen DAB species were demonstrated to be antagonists against the M. incognita–P. nicotianae complex. Because S. flavofungini SNA26, P. putida SNB53 and S. marcescens subsp. sakuensis SNB54 significantly suppressed the infection of M. incognita and P. nicotianae in tobacco, these species have potential for development as biocontrol agents against the diseases and complex caused by these two pathogens. © 2014 Society of Chemical Industry