Special issue devoted to contributions presented at the E-MRS Symposium C ‘Peptide-based materials: from nanostructures to applications’, 7–11 June 2010, Strasbourg, France.
A new family of peptide–nucleic acid nanostructures with potent transfection activities†
Article first published online: 23 NOV 2010
Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.
Journal of Peptide Science
Special Issue: Peptide-based materials: from nanostructures to applications
Volume 17, Issue 2, pages 88–93, February 2011
How to Cite
Bechinger, B., Vidovic, V., Bertani, P. and Kichler, A. (2011), A new family of peptide–nucleic acid nanostructures with potent transfection activities. J. Peptide Sci., 17: 88–93. doi: 10.1002/psc.1318
- Issue published online: 13 JAN 2011
- Article first published online: 23 NOV 2010
- Manuscript Revised: 22 SEP 2010
- Manuscript Accepted: 22 SEP 2010
- Manuscript Received: 21 JUL 2010
- Vaincre la Mucoviscidose
- Agence Nationale de la Recherche
- solid-state NMR;
- isothermal titration calorimetry;
- histidine-rich peptide;
- antimicrobial peptide;
- membrane interaction;
- cell-penetrating peptide
A family of His-rich peptides has been shown to complex DNA and efficiently deliver these nucleic acids into eukaryotic cells. Therefore, these nanoscale complexes have potential applications in gene therapy. Here, we review a number of spectroscopic and biophysical investigations aimed at characterizing the supramolecular interactions of the peptides with the nucleic acids and when overcoming the membrane barriers of the cell. Furthermore, solid-state NMR distance measurements for the first time reveal close interatomic distances between the amino acid side chains and the DNA phosphates within the transfection complex. A recent study indicates that the peptides are also potent transfectants of siRNAs and they could thereby be of potential interest for gene silencing therapies using these compounds. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.