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Synthesis and self-assembly of a neoglycopeptide: morphological studies and ultrasound-mediated DNA encapsulation

Authors

  • Nidhi Gour,

    1. Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208 016 UP, India
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    • Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400005, India.

  • Sudipta Mondal,

    1. Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208 016 UP, India
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  • Sandeep Verma

    Corresponding author
    1. Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208 016 UP, India
    • Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208 016 UP, India.
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  • Special issue devoted to the E-MRS Symposium C “Peptide-based materials: from nanostructures to applications”, 7–11 June 2010, Strasbourg, France.

Abstract

Self-assembly in peptides and proteins is an often encountered concept, where constituent building blocks are recruited and stabilized, via carefully orchestrated hydrophobic interactions, hydrogen bonding and other non-covalent interactions, to eventually reveal an array of supramolecular aggregates, with defined structural features. This study presents synthesis and self-assembly of a mannosylated peptide in aqueous medium. Turbidimetric assay with Concanavalin A (Con A), a mannose binding protein, was conducted to confirm the presence of hydrophilic mannose group on the exterior surface of self-assembled structures. DNA encapsulation in these soft structures was achieved by ultrasonication of soft spherical structures in the presence of plasmid DNA. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.

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