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A novel synthetic peptide from a tomato defensin exhibits antibacterial activities against Helicobacter pylori

Authors

  • M. M. Rigano,

    1. University of Naples ‘Federico II’, School of Biotechnological Sciences, Department of Soil, Plant, Environmental and Animal Production Sciences, Portici, Italy
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  • A. Romanelli,

    1. University of Naples ‘Federico II’, School of Biotechnological Sciences, Department of Biological Sciences, Naples, Italy
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  • A. Fulgione,

    1. University of Naples ‘Federico II’, School of Biotechnological Sciences, Department of Soil, Plant, Environmental and Animal Production Sciences, Portici, Italy
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  • N. Nocerino,

    1. University of Naples ‘Federico II’, School of Biotechnological Sciences, Department of Soil, Plant, Environmental and Animal Production Sciences, Portici, Italy
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  • N. D'Agostino,

    1. CRA-ORT, Agricultural Research Council, Research Centre for Vegetable Crops, Pontecagnano, SA, Italy
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  • C. Avitabile,

    1. University of Naples ‘Federico II’, School of Biotechnological Sciences, Department of Biological Sciences, Naples, Italy
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  • L. Frusciante,

    1. University of Naples ‘Federico II’, Department of Soil, Plant, Environmental and Animal Production Sciences, Portici, Italy
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  • A. Barone,

    1. University of Naples ‘Federico II’, School of Biotechnological Sciences, Department of Soil, Plant, Environmental and Animal Production Sciences, Portici, Italy
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  • F. Capuano,

    1. Department of Food Inspection IZS ME, Portici, Italy
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  • R. Capparelli

    Corresponding author
    • University of Naples ‘Federico II’, School of Biotechnological Sciences, Department of Soil, Plant, Environmental and Animal Production Sciences, Portici, Italy
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Rosanna Capparelli, University of Naples ‘Federico II’, School of Biotechnological Sciences, Department of Soil, Plant, Environmental and Animal Production Sciences, Via Università 100, 80055 Portici, Naples, Italy. E-mail: capparel@unina.it

Abstract

Defensins are a class of cysteine-rich proteins, which exert broad spectrum antimicrobial activity. In this work, we used a bioinformatic approach to identify putative defensins in the tomato genome. Fifteen proteins had a mature peptide that includes the well-conserved tetradisulfide array. We selected a representative member of the tomato defensin family; we chemically synthesized its γ-motif and tested its antimicrobial activity. Here, we demonstrate that the synthetic peptide exhibits potent antibacterial activity against Gram-positive bacteria, such as Staphylococcus aureus A170, Staphylococcus epidermidis, and Listeria monocytogenes, and Gram-negative bacteria, including Salmonella enterica serovar Paratyphi, Escherichia coli, and Helicobacter pylori. In addition, the synthetic peptide shows minimal (<5%) hemolytic activity and absence of cytotoxic effects against THP-1 cells. Finally, SolyC exerts an anti-inflammatory activity in vitro, as it downregulates the level of the proinflammatory cytokines TNF-α and IFN-γ. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd.

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