A general synthetic method toward uridylylated picornavirus VPg proteins


Correspondence to: Dmitri V. Filippov, Leiden Institute of Chemistry, Leiden University, P. O. Box 9502, 2300 RA Leiden, The Netherlands. E-mail: filippov@chem.leidenuniv.nl


Small proteins called viral protein genome-linked (VPg), attached to the 5′-end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre-uridylylated tyrosine building block in an on-line solid phase-based approach. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.