Production and characterization of recombinant human beta-defensin DEFB120

Authors

  • Haiyan Liu,

    1. The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
    2. School of Life Science and Technology, China Pharmaceutical University, Nanjing, China
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  • Heguo Yu,

    1. NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, China
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  • Aijie Xin,

    1. NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, China
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  • Huijuan Shi,

    1. NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, China
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  • Yihua Gu,

    1. NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, China
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  • Yonglian Zhang,

    1. NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, China
    2. Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
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  • Hua Diao,

    Corresponding author
    1. NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, China
    • Correspondence to: Hua Diao, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.

      E-mail: diaohua@gmail.com.

      Correspondence to: Donghai Lin, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

      E-mail: dhlin@xmu.edu.cn

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  • Donghai Lin

    Corresponding author
    1. The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
    2. School of Life Science and Technology, China Pharmaceutical University, Nanjing, China
    • Correspondence to: Hua Diao, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.

      E-mail: diaohua@gmail.com.

      Correspondence to: Donghai Lin, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

      E-mail: dhlin@xmu.edu.cn

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Abstract

Public health of human beings is threatened by superbugs. Novel human beta-defensins, which contribute to host defense against pathogen invasion and innate immune protection, might be a potent natural candidate pool for new antibiotic lead screening. In the present work, we successfully expressed and purified a novel human beta-defensin, DEFB120, using the IMPACT-TWIN system in Escherichia coli and identified the purified homogeneous proteins using MALDI-TOF mass spectrometry. Then, we performed the fundamental studies on the structure and biological functions for the DEFB120 peptide. The recombinant DEFB120 peptide showed wide antimicrobial effects against E. coli, Staphylococcus aureus and Candida albicans strains without significant hemolytic activity. Furthermore, the high lipopolysaccharide (LPS)-binding affinity in vitro indicated that DEFB120 might be associated with the inhibition of LPS-induced inflammatory response. These results may pave a way for exploiting the essential physiological functions of DEFB120 and also for the development of natural antibiotic pools. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd.

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