Functional properties of a novel hybrid antimicrobial peptide NS: potent antitumor activity and efficient plasmid delivery

Authors

  • Yun Zhang,

    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China
    2. School of Pharmacy, Lanzhou University, Lanzhou, China
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    • These authors contributed equally to this work

  • Jingjing Song,

    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China
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    • These authors contributed equally to this work

  • Wei Zhang,

    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China
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  • Ranran Liang,

    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China
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  • Yinyun Ma,

    1. School of Pharmacy, Lanzhou University, Lanzhou, China
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  • Li Zhang,

    1. School of Pharmacy, Lanzhou University, Lanzhou, China
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  • Xiaojin Wei,

    1. School of Pharmacy, Lanzhou University, Lanzhou, China
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  • Jingman Ni,

    Corresponding author
    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China
    2. School of Pharmacy, Lanzhou University, Lanzhou, China
    • Correspondence to: Rui Wang and Jingman Ni, Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences and School of Pharmacy, Lanzhou University, Lanzhou 730000, China. E-mail: wangrui@lzu.edu.cn; nijm@lzu.edu.cn

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  • Rui Wang

    Corresponding author
    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China
    • Correspondence to: Rui Wang and Jingman Ni, Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences and School of Pharmacy, Lanzhou University, Lanzhou 730000, China. E-mail: wangrui@lzu.edu.cn; nijm@lzu.edu.cn

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Abstract

Antimicrobial peptides have been widely recognized as potential candidates for treating tumor, especially for defending against multidrug-resistant cells. Previously, based on the structure of substance P, we have designed a novel class of hybrid antimicrobial peptide NS, which possesses potent antimicrobial activity against a broad spectrum of bacterial pathogens. In this study, we evaluated its cytotoxicity to tumor cells and studied the possible mechanism of action. We showed that NS could efficiently kill tumor cells by rapidly disrupting the tumor cell membrane and inhibiting the DNA synthesis. In addition, we also found that NS could efficiently deliver plasmids into cells and exhibit high transfection efficiency after the introduction of a stearyl moiety to its N-terminus, like many reported cell-penetrating peptides. Taken together, this study revealed the potential multiple functions of NS, providing fundamental support for further therapeutic application as potential antitumor agent. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd.

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