Synthesis of a New Template with a Built-in Adjuvant and Its Use in Constructing Peptide Vaccine Candidates Through Polyoxime Chemistry
Article first published online: 4 DEC 1998
Copyright © 1996 European Peptide Society and John Wiley & Sons, Ltd.
Journal of Peptide Science
Volume 2, Issue 1, pages 66–72, January 1996
How to Cite
Zeng, W., Jackson, D. C. and Rose, K. (1996), Synthesis of a New Template with a Built-in Adjuvant and Its Use in Constructing Peptide Vaccine Candidates Through Polyoxime Chemistry. J. Peptide Sci., 2: 66–72. doi: 10.1002/psc.51
- Issue published online: 4 DEC 1998
- Article first published online: 4 DEC 1998
- Manuscript Accepted: 12 OCT 1995
- Manuscript Received: 20 SEP 1995
- polypeptide vaccine
Synthetic lipopeptides are showing promise as vaccine candidates, but until now it has been very difficult to prepare them in homogeneous form. We describe the synthesis and characterization of a new water-soluble, four-branched template with a built-in lipophilic adjuvant (Pam3Cys). Through the use of oxime chemistry, we attached four copies of an unprotected influenza virus peptide and characterized the product (13kDa) by reversed-phase HPLC and electrospray ionization mass spectrometry. Several other such constructions were made using the new template and different peptides. We seem to have a general method for making synthetic lipopeptides in homogeneous form.