Journal of Peptide Science

Cover image for Vol. 17 Issue 1

January 2011

Volume 17, Issue 1

Pages 1–72

  1. Protocols

    1. Top of page
    2. Protocols
    3. Research Articles
    1. Optimization of oxidative folding methods for cysteine-rich peptides: a study of conotoxins containing three disulfide bridges (pages 1–7)

      Andrew M. Steiner and Grzegorz Bulaj

      Article first published online: 2 SEP 2010 | DOI: 10.1002/psc.1283

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      The oxidative folding of small, cysteine-rich peptides to selectively achieve the native disulfide bond connectivities is critical for discovery and structure-function studies of many bioactive peptides. Because the propensity to acquire the native conformation greatly depends on the peptide sequence, numerous empirical oxidation methods are employed. Herein we compare the efficacy of optimized solution-phase and polymer-supported oxidation methods using three-disulfide bridged conotoxins. We propose the ClearOx-assisted oxidation of selenopeptides as a fairly generalized oxidative folding protocol.

    2. Facile synthesis of peptide nucleic acids and peptide nucleic acid-peptide conjugates on an automated peptide synthesizer (pages 8–13)

      Rajendra Joshi, Deepti Jha, Wu Su and Joern Engelmann

      Article first published online: 25 OCT 2010 | DOI: 10.1002/psc.1305

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      A facile protocol for the fully automated or semi-automated synthesis of PNAs or PNA-peptide conjugates is presented.

  2. Research Articles

    1. Top of page
    2. Protocols
    3. Research Articles
    1. Interactions of amyloid Aβ(1–42) peptide with self-assembled peptide nanospheres (pages 14–23)

      Evan M. Smoak, Melanie P. Dabakis, Marsiyana M. Henricus, Robert Tamayev and Ipsita A. Banerjee

      Article first published online: 2 SEP 2010 | DOI: 10.1002/psc.1284

      Thumbnail image of graphical abstract

      The interactions of Aβ(1–42) peptide with self-assembled peptide nanospheres was studied. It was found that at low concentrations, a relative diminution in the aggregation of Aβ(1–42) was observed. Further, the interactions were concentration as well as pH dependent.

    2. Peptide vaccine candidates against classical swine fever virus: T cell and neutralizing antibody responses of dendrimers displaying E2 and NS2–3 epitopes (pages 24–31)

      Marta Monsó, Joan Tarradas, Beatriz G. de la Torre, Francisco Sobrino, Llilianne Ganges and David Andreu

      Article first published online: 2 SEP 2010 | DOI: 10.1002/psc.1292

      Thumbnail image of graphical abstract

      Dendrimeric constructs integrating four B and one T epitopes of classical swine fever virus have been used for immunization of domestic pigs, to evaluate the protective response induced and to characterize the B- and T-cell response against the virus in the natural host. For synthesis of the dendrimeric platforms, stepwise SPPS using 3,6-dioxaoctanoic acid as flexible spacer at the branching points is clearly advantageous over ligation in solution.

    3. Improved Fmoc-based solid-phase synthesis of homologous peptide fragments of human and mouse prion proteins (pages 32–38)

      Dolors Grillo-Bosch, Francesc Rabanal and Ernest Giralt

      Article first published online: 16 SEP 2010 | DOI: 10.1002/psc.1293

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      The synthesis of difficult peptide sequences has been a challenge since the very beginning of SPPS. We propose an appropriate combination of resin and an optimised coupling strategy to yield peptides containing difficult sequences prone to aggregate in high yield and purity.

    4. Efficient synthesis of an (aminooxy) acetylated-somatostatin derivative using (aminooxy)acetic acid as a ‘carbonyl capture’ reagent (pages 39–46)

      Gábor Mezö, Ildikó Szabó, István Kertész, Rózsa Hegedüs, Erika Orbán, Ulrike Leurs, Szilvia Bösze, Gábor Halmos and Marilena Manea

      Article first published online: 2 SEP 2010 | DOI: 10.1002/psc.1294

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      An (aminooxy)acetylated-somatostatin derivative was efficiently synthesized in the presence of free (aminooxy)acetic acid, which was used as a ‘carbonyl capture’ reagent in the final cleavage step.

    5. A retro-inverso α-melanocyte stimulating hormone analog with MC1R-binding selectivity (pages 47–55)

      Timothy Weeden, Jim Stefano, Su Duan, Andrea Edling, Lihui Hou, Wei-Lien Chuang, Michael A. Perricone, Clark Pan and John L. Dzuris

      Article first published online: 25 OCT 2010 | DOI: 10.1002/psc.1306

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      A retro-inverso analog of alpha-melanocyte stimulating hormone (RI-α-MSH) that is proteolytically stable and highly selective for the melanocortin 1 receptor (MC1R).

    6. Tetrad selectivity in polarity-driven switch peptides: the best turn is not always the best nucleation site (pages 56–67)

      Katja Gehenn and Jennifer Reed

      Article first published online: 27 SEP 2010 | DOI: 10.1002/psc.1296

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      We identified new naturally occurring families of polarity-driven conformational switch peptides. Moreover, we were able to refine the physico-chemical characteristics of N-terminal helix–inducing tetrads within these peptides. The work presented here should contribute to significant advancement in our understanding of the basic principles of this unusual folding behaviour and could provide a deeper view into the mechanism of protein–protein or protein–membrane interactions and extends our knowledge of cellular and extra-cellular recognition processes.

    7. Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata (pages 68–72)

      Xiuhong Liu, Rui Liu, Lin Wei, Hailong Yang, Keyun Zhang, Jingze Liu and Ren Lai

      Article first published online: 25 OCT 2010 | DOI: 10.1002/psc.1309

      Purification of antimicrobial peptides brevinin-2-RN1 and-RN2. The amino acid and cDNA sequences of brevinin-2RN1 and - RN2. The antimicrobial activities of the two novel antimicrobial peptides.

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