Development of [Ile40]HTLV-I protease inhibition assay using novel fluorogenic and chromogenic substrate (pages 569–575)
Henri-Obadja Kumada, Jeffrey-Tri Nguyen, Taeko Kakizawa, Koushi Hidaka, Tooru Kimura, Yoshio Hayashi and Yoshiaki Kiso
Article first published online: 13 MAY 2011 | DOI: 10.1002/psc.1375
In HTLV-I protease assay, a bifunctional fluorogenic and chromogenic substrate was designed, synthesized and found to enhance both UV and fluorescence detection over a conventional mono-functional chromogenic substrate. A newly established HTLV-I protease inhibition assay using a stable L40I mutant HTLV-I protease and the fluorescent substrate requires distinctively less protease, substrate, inhibitor and assay time than the previous methods. The assay is more cost-effective and time-efficient while being reproducible and less labor-intensive.