Journal of Peptide Science

Cover image for Vol. 18 Issue 2

February 2012

Volume 18, Issue 2

Pages 73–145

  1. Reviews

    1. Top of page
    2. Reviews
    3. Research Articles
    1. The good taste of peptides (pages 73–82)

      Piero A. Temussi

      Article first published online: 7 DEC 2011 | DOI: 10.1002/psc.1428

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      This review describes the main peptides with a sweet, umami or bitter taste and their relationship with food acceptance or rejection. Particular emphasis is given to the sweet taste modality, owing to the practical and scientific relevance of aspartame and the theoretical importance of sweet proteins. The validation of the wedge model for sweet proteins gives confidence in the reliability of the homology model of the T1R2-T1R3 receptor itself and allows its use to explain some aspects of aspartame interactions.

  2. Research Articles

    1. Top of page
    2. Reviews
    3. Research Articles
    1. Detection of nonopioid β-endorphin receptor in the rat myocardium (pages 83–87)

      Yulia N. Nekrasova, Yury A. Zolotarev and Elena V. Navolotskaya

      Article first published online: 3 NOV 2011 | DOI: 10.1002/psc.1417

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      Two synthetic peptides TPLVTLFK (octarphin) and SLTCLVKGFY (immunorphin) were found to bind to high-affinity naloxone-insensitive binding sites on the membranes isolated from the rat myocardium (Kd = 2.0 ± 0.2 and 2.5 ± 0.3 nM, respectively). The [3H]octarphin specific binding to the myocardium was inhibited by unlabeled β-endorphin (Ki = 1.9 ± 0.2 nM) and immunorphin (Ki = 2.2 ± 0.3 nM), and the [3H]immunorphin specific binding was inhibited by by unlabeled β-endorphin (Ki = 2.3 ± 0.3 nM) and octarphin (Ki = 2.4 ± 0.3 nM). In both cases, these binding sites were insensitive to naloxone and agonists of opioid receptors.

    2. Fast conventional Fmoc solid-phase peptide synthesis: a comparative study of different activators (pages 88–91)

      Christina Ann Chantell, Michael Abayomi Onaiyekan and Mahendra Menakuru

      Article first published online: 7 DEC 2011 | DOI: 10.1002/psc.1419

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      A panel of activators (COMU, HDMC, HCTU, HATU, PyOxim, PyClock, PyBOP, and TFFH) are evaluated for their ability to perform fast solid-phase peptide synthesis.

    3. Antinociceptive effect of [Met5]enkephalin semicarbazide is not affected by dipeptidyl carboxypeptidase-I (pages 92–96)

      Zahra Rezaee, Seyed Abbas Arabanian, Saeed Balalaie, Abolhassan Ahmadiani, Leila Khalaj and Sanaz Nasoohi

      Article first published online: 14 NOV 2011 | DOI: 10.1002/psc.1420

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      Antinociceptive effects of two new derivatives of [Met5]enkephalin (R = ethylamide or semicarbazide) were evaluated by tail flick test. A mixture of three inhibitors was used to protect the enkephalin analogues from the relevant peptidases biodegradation. Antinociceptions produced by the enkephalin analogues were compared after omitting captopril from the peptidase inhibitors mixture.

    4. Antibacterial potential of hGlyrichin encoded by a human gene (pages 97–104)

      Jibin Sha, Guang Zhao, Xiaojuan Chen, Weiping Guan, Yanling He and Zhaoqing Wang

      Article first published online: 14 NOV 2011 | DOI: 10.1002/psc.1421

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      The hGlyrichin polypeptide kills a variety of bacteria including the multidrug-resistant (MDR) bacteria through the destruction of the bacterial membrane. A 19 amino acid peptide (pCM19) at position 42-60 of hGlyrichin is crucial for its antibacterial activity. In addition, all peptides that are homologous to hGlyrichin have antibacterial activity and can penetrate the bacterial membrane.

    5. Comparative activity and mechanism of action of three types of bovine antimicrobial peptides against pathogenic Prototheca spp. (pages 105–113)

      Linda Tomasinsig, Barbara Skerlavaj, Michele Scarsini, Filomena Guida, Renata Piccinini, Alessandro Tossi and Margherita Zanetti

      Article first published online: 14 NOV 2011 | DOI: 10.1002/psc.1422

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      Three structurally diverse antimicrobial peptides (BMAP-28, Bac5, LAP) show the ability to inactivate pathogenic Prototheca spp: the activity of BMAP-28 is based on rapid membrane permeabilization, whereas Bac5 and LAP act without causing membrane damage. Accordingly, the conformation of BMAP-28, but not that of Bac5 or LAP, is affected by interaction with liposomes mimicking Prototheca membranes. Our results suggest that these peptides are appropriate for protothecal mastitis treatment.

    6. Synthesis and NMR elucidation of novel tetrapeptides (pages 114–121)

      Maya Makatini, Thashini Chetty, Oluseye K. Onajole, Thavendran Govender, Patrick Govender, Glenn E. M. Maguire and Hendrik G. Kruger

      Article first published online: 11 NOV 2011 | DOI: 10.1002/psc.1423

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      The synthesis and NMR elucidation of Ala-Val-Pro-Ile and five novel peptide-based derivatives that mimic the natural second mitochondria-derived activator of caspase (Smac) protein are reported. The use of 2D NMR techniques with the inclusion of efficient adiabatic symmetrized ROESY proves to be effective tools to elucidate the structures.

    7. Effect of introducing a short amyloidogenic sequence from the Aβ peptide at the N-terminus of 18-residue amphipathic helical peptides (pages 122–128)

      Chandrasekaran SivakamaSundari, Sridharan Rukmani and Ramakrishnan Nagaraj

      Article first published online: 2 NOV 2011 | DOI: 10.1002/psc.1424

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      Presence of Aβ16–22 sequence KLVFFAE at the N-terminus of two amphipathic helical 18-residue peptides induces fibrillization only when the net charge is zero.

    8. Solution structure by nuclear magnetic resonance of the two lantibiotics 97518 and NAI-107 (pages 129–134)

      Francesca Vasile, Donatella Potenza, Barbara Marsiglia, Sonia Maffioli and Stefano Donadio

      Article first published online: 14 NOV 2011 | DOI: 10.1002/psc.1425

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      High-resolution 3D structures of two lantibiotics (97518 and NAI-107) were obtained by NMR and molecular dynamics simulations. Despite their similar structure, the two lantibiotics exhibit significant differences in their potency. They represent promising compounds to treat infections caused by multiresistant Gram-positive pathogens.

    9. Ubiquitination of an artificial RING finger without a substrate and a tag (pages 135–139)

      Kazuhide Miyamoto

      Article first published online: 24 NOV 2011 | DOI: 10.1002/psc.1426

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      The present study provides the method ′α-helical region substitution' for the creation of the artificial ubiquitin-ligating enzyme (E3). The WSTF PHD finger is not able to ubiquitinate upon itself; however, the method enables it to possess the ubiquitination capability for functioning as E3. The WSTF PHD_EL5 and WSTF PHD_SIAH1 RING fingers possess E3 activities of poly-ubiquitination and mono-ubiquitination, respectively.

    10. The solution structure of the S4–S5 linker of the hERG potassium channel (pages 140–145)

      Shovanlal Gayen, Qingxin Li and CongBao Kang

      Article first published online: 3 NOV 2011 | DOI: 10.1002/psc.1427

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      The homology model of the channel domain of the hERG potassium channel is shown. Residues possibly interacting with the cell membrane are shown in stick mode. The electrostatic potential surface representation of the S4–S5 linker in dodecylphosphocholine micelles indicated the possible residues facing the cell membrane.

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