Journal of Peptide Science

Cover image for Vol. 18 Issue 6

June 2012

Volume 18, Issue 6

Pages 357–426

  1. Reviews

    1. Top of page
    2. Reviews
    3. Research Articles
    1. A structural view on spider silk proteins and their role in fiber assembly (pages 357–365)

      Franz Hagn

      Version of Record online: 8 MAY 2012 | DOI: 10.1002/psc.2417

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      Spider silk is the toughest known biomaterial and even outrivals modern synthetic high-performance materials. Here, the focus is on the structure and function of the conserved N- and C-terminal domains of spider dragline silk proteins which, unlike the very long repetitive sequence elements, adopt a folded conformation in solution and are therefore able to control intermolecular interactions and aggregation between other silk molecules. These two domains sensor pH, salts, and shear stress and use these stimuli to induce controlled fiber assembly.

  2. Research Articles

    1. Top of page
    2. Reviews
    3. Research Articles
    1. Surface immobilization chemistry influences peptide-based detection of lipopolysaccharide and lipoteichoic acid (pages 366–372)

      Stella H. North, Jason Wojciechowski, Virginia Chu and Chris R. Taitt

      Version of Record online: 4 MAY 2012 | DOI: 10.1002/psc.2399

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      The functional binding activity of surface-bond AMPs is highly sensitive to the choice of immobilization chemistry used to covalently attach peptides to microtiter plates. Two analogous amine-reactive immobilization chemistries can result in significant differences in peptide-specific immobilization efficiency as well as the presentation/orientation of surface-bound peptides. These qualities translate into significant differences in the ability of the immobilized peptides to differentially recognize and bind to LPS and LTA.

    2. Interaction structure of the complex between neuroprotective factor humanin and Alzheimer's β-amyloid peptide revealed by affinity mass spectrometry and molecular modeling (pages 373–382)

      Madalina Maftei, Xiaodan Tian, Marilena Manea, Thomas E. Exner, Daniel Schwanzar, Christine A. F. von Arnim and Michael Przybylski

      Version of Record online: 20 APR 2012 | DOI: 10.1002/psc.2404

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      The interaction epitopes between the neuroprotective peptide humanin and ß-amyloid(1–40) were identified to reside in the humanin(5–15) and Aß(17–28) sequences by affinity mass spectrometry, using proteolytic excision and extraction. The interaction structure was ascertained and characterized by mass spectrometry, molecular dynamics simulation and affinity quantification. The interaction epitopes and specific association of humanin with ß-amyloid provide a molecular basis for understanding the neuroprotective function of humanin and for the design of new therapeutic peptides.

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      Restoration of leptin responsiveness in diet-induced obese mice using an optimized leptin analog in combination with exendin-4 or FGF21 (pages 383–393)

      Timo D. Müller, Lorraine M. Sullivan, Kirk Habegger, Chun-Xia Yi, Dhiraj Kabra, Erin Grant, Nickki Ottaway, Radha Krishna, Jenna Holland, Jazzminn Hembree, Diego Perez-Tilve, Paul T. Pfluger, Michael J. DeGuzman, Marc E. Siladi, Vadim S. Kraynov, Douglas W. Axelrod, Richard DiMarchi, Jason K. Pinkstaff and Matthias H. Tschöp

      Version of Record online: 4 MAY 2012 | DOI: 10.1002/psc.2408

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      This study reports the biosynthesis of a series of optimized leptin analogs with sustained duration of in vivo action. Beyond the synthesis and site-specific PEGylation, we report the sustained in vivo action and in vivo profile of single treatment of diet-induced obesity, as well as in combination with other weight loss-inducing macromolecules. Our results show that leptin sensitivity can be restored by more than one polypharmacy strategy and that this phenomenon does not depend on a specific signaling pathway.

    4. Determination of protease subsite preference on SPOT peptide array by fluorescence quenching-based assay (pages 394–399)

      Do-Hyun Kim, Dong-Sik Shin and Yoon-Sik Lee

      Version of Record online: 29 APR 2012 | DOI: 10.1002/psc.2409

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      A mixture-based peptide SPOT array was synthesized and used to determine protease subsite preference. The peptide substrates on the array were hydrolyzed by thrombin to screen the subsite specificity by a fluorescence quenching-based assay.

    5. Influence of lithium cations on prolyl peptide bonds (pages 400–404)

      Claudia Kunz, Günther Jahreis, Robert Günther, Stefan Berger, Gunter Fischer and Hans-Jörg Hofmann

      Version of Record online: 20 APR 2012 | DOI: 10.1002/psc.2410

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      Lithium cations influence considerably the cis–trans equilibrium of prolyl peptide bonds in solvents like TFE. This study examines systematically the role of environmental and structural aspects by NMR spectroscopy.

    6. Controlling morphology of peptide-based soft structures by covalent modifications (pages 405–412)

      Nidhi Gour, Apurba K. Barman and Sandeep Verma

      Version of Record online: 25 APR 2012 | DOI: 10.1002/psc.2411

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      This report describes morphological changes, from tubular morphology to spherical structures, in diphenylalanine self-assembled structures upon mannose conjugation.

    7. Structure of a novel PTH-related peptide hPTH′ and its interaction with the PTH receptor (pages 413–417)

      Kejiang Lin, Yonggan Len, Jao Feng, Hongchang Gao, Qidong You, Donghai Lin and Jingjing Liu

      Version of Record online: 19 APR 2012 | DOI: 10.1002/psc.2412

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      We characterized the solution conformations of hPTH′ by NMR spectroscopy and modeled the interactions between hPTH′ and PTH receptor. By comparing it with PTH (1–34) structures, we found that mutated arginine makes hPTH′ fill the receptor cavity better as well as forms hydrogen bonds with Val193.

    8. Synthesis and biological evaluation of new active For-Met-Leu-Phe-OMe analogues containing para-substituted Phe residues (pages 418–426)

      Adriano Mollica, Federica Feliciani, Azzurra Stefanucci, Roberto Costante, Gino Lucente, Francesco Pinnen, Daniela Notaristefano and Susanna Spisani

      Version of Record online: 24 APR 2012 | DOI: 10.1002/psc.2414

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      Synthesis and biological evaluation of novel N-Boc-protected tripeptides 4a–l and N-For protected tripeptides 5a–l as new For-Met-Leu-Phe-OMe analogues are reported.