Journal of Peptide Science

Cover image for Vol. 18 Issue 8

August 2012

Volume 18, Issue 8

Pages 487–540

  1. Research Articles

    1. Top of page
    2. Research Articles
    1. Homology, similarity, and identity in peptide epitope immunodefinition (pages 487–494)

      Darja Kanduc

      Version of Record online: 13 JUN 2012 | DOI: 10.1002/psc.2419

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      Using a proper measurement unit and perfect peptide matching constrain sequence similarity analysis in immunology. A correct unit may be represented by five residues, and sequence similarity needs to be measured by percent identity, because amino acid substitutions, biochemical similarities, or posttranslational modifications are tolerated with difficulty or not at all in the immunological context.

    2. Synthetic peptide TPLVTLFK (octarphin) reduces the corticosterone production by rat adrenal cortex through nonopioid β-endorphin receptor (pages 495–499)

      Yuliia N. Nekrasova, Yury A. Zolotarev and Elena V. Navolotskaya

      Version of Record online: 29 JUN 2012 | DOI: 10.1002/psc.2424

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      The synthetic peptide octarphin (TPLVTLFK) labeled with tritium was found to bind to high-affinity nonopioid β-endorphin receptors on rat adrenal cortex membranes (Kd = 35.7 ± 2.3 nM). Octarphin at concentrations of 10−9–10−6 M was found to inhibit the adenylate cyclase activity in adrencortical membranes, whereas intranasal injection of octarphin was found to reduce the secretion of corticosterone from the adrenals to the bloodstream. Thus, octarphin decreases the adrenal cortex functional activity through the nonopioid receptor of β-endorphin.

    3. Peptaibols from two unidentified fungi of the order Hypocreales with cytotoxic, antibiotic, and anthelmintic activities (pages 500–510)

      Sloan Ayers, Brandie M. Ehrmann, Audrey F. Adcock, David J. Kroll, Esperanza J. Carcache de Blanco, Qi Shen, Steven M. Swanson, Joseph O. Falkinham III, Mansukh C. Wani, Sheila M. Mitchell, Cedric J. Pearce and Nicholas H. Oberlies

      Version of Record online: 29 JUN 2012 | DOI: 10.1002/psc.2425

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      Fifteen peptaibols were isolated and characterized from two different fungal cultures; eight are new to the literature. The compounds were sequenced by MS/MS using higher-energy collisional dissociation and high field NMR (950 MHz), both of which facilitated structure elucidation, and the absolute configuration of the amino acid residues was determined using Marfey's analysis via the development of a 10-min ultra performance liquid chromatography method. The compounds were examined against a suite of biological assays, including cytotoxicity, antibacterial activity, and anthelmintic activity.

    4. Novel approach for optimization of a ‘difficult’ peptide synthesis by utilizing quantitative reaction monitoring assays (pages 511–518)

      Jari I. Finneman and Mark J. Pozzo

      Version of Record online: 4 JUL 2012 | DOI: 10.1002/psc.2428

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      Use of quantitative reaction monitoring assays is illustrated in optimization of ‘difficult’ EGFRvIII peptide by solid-phase peptide synthesis. Optimized process increased the crude peptide purity to approximately 10% compared with the standard approaches.

    5. Stability, toxicity and biological activity of retro, inversed and retro-inversed glucagon isomers (pages 519–526)

      Besnik Krasniqi, Erin Scruten, Jaret Piller, Jeremy Lee and Scott Napper

      Version of Record online: 3 JUL 2012 | DOI: 10.1002/psc.2429

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      Retro, inversed and retro-inversed isomers of glucagon were investigated with respect to structure, stability, toxicity and biological activity. Biologically, retro-inversed glucagon demonstrated comparable in vivo activity as L-glucagon. Structurally circular dichroism indicated that the directional and chiral isomers adopted related structures, nanopore analysis discriminated structural behaviors unique to each isomer.

    6. Peptide-mediated cell penetration and targeted delivery of gold nanoparticles into lysosomes (pages 527–534)

      Chaitali D. Dekiwadia, Ann C. Lawrie and John V. Fecondo

      Version of Record online: 4 JUL 2012 | DOI: 10.1002/psc.2430

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      The delivery of 13-nm gold nanoparticles across the cell membrane followed by targeted localisation into the lysosomes of a mammalian cell line was examined using novel combinations of cell-penetrating peptides and lysosomal sorting peptides conjugated to the nanoparticles. Using a combination of fluorescence spectroscopy, fluorescence microscopy and transmission electron microscopy techniques, we show that these nanoconjugates were efficiently and selectively delivered into the lysosomes with minimal cytotoxic effects.

    7. Design and synthesis of tryptophan containing peptides as potential analgesic and anti-inflammatory agents (pages 535–540)

      R. Suhas and D. Channe Gowda

      Version of Record online: 9 JUL 2012 | DOI: 10.1002/psc.2431

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      This article focuses on the identification of novel group of peptides as potential analgesic and anti-inflammatory agents; their synthesis, characterization and pharmacological evaluation have been described.