Journal of Peptide Science

Cover image for Vol. 19 Issue 1

January 2013

Volume 19, Issue 1

Pages 1–63

  1. Research Articles

    1. Top of page
    2. Research Articles
    1. Antibacterial and membrane-damaging activities of β-bungarotoxin B chain (pages 1–8)

      Yi-Lin Wen, Bao-Jueng Wu, Pei-Hsiu Kao, Yaw-Syan Fu and Long-Sen Chang

      Article first published online: 8 NOV 2012 | DOI: 10.1002/psc.2463

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      The antibacterial action of the B chain is related to its ability to induce membrane permeability. Destabilization of the lipopolysaccharide layer and inhibition of lipoteichoic acid biosynthesis on cell wall increased the bactericidal effect of the B chain on Escherichia coli and Staphylococcus aureus.

    2. Solid phase synthesis of peptide hydroxamic acids on poly(ethylene glycol)-based support (pages 9–15)

      Marta Cal, Mariusz Jaremko, Łukasz Jaremko and Piotr Stefanowicz

      Article first published online: 5 DEC 2012 | DOI: 10.1002/psc.2466

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      A novel resin designed for solid-phase synthesis of peptide hydroxamic acids (PHA) combining the trityl linker with poly(ethylene glycol)-based support, ChemMatrix® type, is described. The synthesis of PHA can be performed according to a standard protocol, providing products in excellent purity and reasonable yields.

    3. Oxidative folding and preparation of α-conotoxins for use in high-throughput structure–activity relationship studies (pages 16–24)

      Reena Gyanda, Jayati Banerjee, Yi-Pin Chang, Angela M. Phillips, Lawrence Toll and Christopher J. Armishaw

      Article first published online: 28 NOV 2012 | DOI: 10.1002/psc.2467

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      The effect of co-solvent, redox reagents, pH, temperature, and time were systematically investigated for α-conotoxins MII, PnIB, GID, AuIB, BuIA, ImI, and GI to determine the optimized conditions for folding to the native disulfide bond isomer. We have also demonstrated an efficient ‘semi-purification’ technique for the high-throughput isolation of α-conotoxins following oxidative folding. Our simplified procedures permit the rapid identification of active hits directly from high-throughput pharmacological screening assays.

    4. Evaluation of new immunological targets in neuromyelitis optica (pages 25–32)

      Jean-Baptiste Chanson, Ilaria Paolini, Nicolas Collongues, Maria C. Alcaro, Frédéric Blanc, Francesca Barbetti, Marie Fleury, Elisa Peroni, Paolo Rovero, Gabrielle Rudolf, Francesco Lolli, Élisabeth Trifilieff, Anna-Maria Papini and Jérôme de Seze

      Article first published online: 28 NOV 2012 | DOI: 10.1002/psc.2470

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      We compared the reactivity against 19 structurally defined peptides in the sera of 26 patients suffering from neuromyelitis optica and 21 healthy subjects. We found that this neurological autoimmune disease is accompanied by an increased reactivity against three peptides. These findings may be used to improve the diagnosis or the comprehension of the disease.

    5. NMR investigations of structural and dynamics features of natively unstructured drug peptide – salmon calcitonin: implication to rational design of potent sCT analogs (pages 33–45)

      Atul Rawat and Dinesh Kumar

      Article first published online: 4 DEC 2012 | DOI: 10.1002/psc.2471

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      The structural and backbone dynamics features of drug peptide salmon calcitonin have been investigated in aqueous solution and have been found to be correlated very well with the functionality of the hormone. As depicted here, the helical sampling by the peptide and significant amount of conformational plasticity towards N-terminal part of the chain may be a reflection of the biologically active state. Further, the peptide in solution exhibits multiple conformational states that in turn may inhibit symmetric self-association of the peptide and thus may account for its reduced aggregation propensity compared with human calcitonin (which lacks this property).

    6. Investigation on chemotactic drug targeting (chemotaxis and adhesion) inducer effect of GnRH-III derivatives in Tetrahymena and human leukemia cell line (pages 46–58)

      Eszter Lajkó, Ildikó Szabó, Katalin Andódy, András Pungor, Gábor Mező and László Kőhidai

      Article first published online: 4 DEC 2012 | DOI: 10.1002/psc.2472

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      The chemotactic drug targeting (CDT) represents a new way for drug delivery approach based on selective chemoattractant guided targeting. In the present study, the GnRH-III derivatives, as potential targeting moieties for CDT, were investigated. The chemoattractant and adhesion inducer effects of GnRH-III, its fragment, and dimer derivatives indicate that these peptides – as carriers and targeting units – might deliver cytotoxic agents directly to the tumor cells by CDT.

    7. Development and clinical application of an enzyme immunoassay for the determination of midregional proadrenomedullin (pages 59–63)

      Yosuke Suzuki, Hiroki Itoh, Fumihiko Katagiri, Fuminori Sato, Yukie Sato, Kanako Kawasaki, Yuhki Sato, Hiromitsu Mimata and Masaharu Takeyama

      Article first published online: 6 DEC 2012 | DOI: 10.1002/psc.2473

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      In this study, we attempted to develop an enzyme immunoassay (EIA) for quantifying mid-regional proadrenomedullin (MR-proADM)-like immunoreactive substance (IS), which is applicable for monitoring plasma MR-proADM levels. Using β-D-galactosidase-labeled preproADM(83-94) as a marker antigen, anti-rabbit IgG-coated immunoplate as a bound/free separator, and 4-methylumbelliferyl-β-D-galactopyranoside as a fluorogenic substrate, a sensitive and specific EIA was developed for the quantification of MR-proADM-IS in human plasma. Our EIA may be useful to evaluate plasma MR-proADM levels as a biomarker in various clinical settings.

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