Journal of Peptide Science

Cover image for Vol. 19 Issue 11

November 2013

Volume 19, Issue 11

Pages 669–724

  1. Research Articles

    1. Top of page
    2. Research Articles
    1. Synthetic analogs of anoplin show improved antimicrobial activities (pages 669–675)

      Jens K. Munk, Lars Erik Uggerhøj, Tanja J. Poulsen, Niels Frimodt-Møller, Reinhard Wimmer, Nils T. Nyberg and Paul R. Hansen

      Article first published online: 9 SEP 2013 | DOI: 10.1002/psc.2548

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      Anoplin, GLLKRIKTLL-NH2, is a decamer amide antimicrobial peptide from wasp venom. We report the effects on hemolytic and bacteriostatic activities of the modifications shown, where X is the cyclohexylalanine of 2-naphthylalanine.

    2. Structure activity relationships of peptidic analogs of MyoD for the development of Id1 inhibitors as antiproliferative agents (pages 676–683)

      Yu-Cheng Hsiao, De-Len Yang, Hui-Ling Hung and Feng-Di T. Lung

      Article first published online: 3 OCT 2013 | DOI: 10.1002/psc.2549

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      SPR results revealed that A-substituted analogs of peptide 3C showed weaker binding to Id1 than that of peptide 3C, indicating the six amino acid residues in the N-terminal of peptide 3C were all essential for binding to Id1 and the importance of amino acid residue was: I2 > Q6 > Y1 > G4 > L5 > E3. In addition, substitution of E3 in peptide 3C with D, Q, and R did not improve the binding potency of peptide 3C. CD results indicated that peptide 3C exhibited the highest content of α-helical structure (39.37%), suggesting that the α-helical structure may contribute to its affinity for Id1 and Id1-HLH.

    3. Effect of TAT-obestatin on proliferation, differentiation, apoptosis and lipolysis in 3T3-L1 preadipocytes (pages 684–691)

      Guangcai Ren, Zuyong He, Peiqing Cong, Jingwei Yu, Yufeng Qin, Yaosheng Chen and Xiaohong Liu

      Article first published online: 14 SEP 2013 | DOI: 10.1002/psc.2550

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      To investigate whether obestatin can interact with intracellular components that activate signalling pathways in adipocytes, we incubated 3T3-L1 cells with a cell-permeable peptide TAT-conjugated obestatin. The results showed that TAT-obestatin can penetrate the cell membrane, and inhibit cell apoptosis and stimulate lipolysis, but have no effect on proliferation and lipogenesis. This suggests the difference between the effect of TAT-obestatin and obestatin on adipocytes metabolism indicated that TAT-obestatin may trigger intracellular signalling as well as signalling at the cell membrane.

    4. Scalable purification of the lantibiotic nisin and isolation of chemical/enzymatic cleavage fragments suitable for semi-synthesis (pages 692–699)

      Jack C. Slootweg, Rob M. J. Liskamp and Dirk T. S. Rijkers

      Article first published online: 11 SEP 2013 | DOI: 10.1002/psc.2551

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      Nisin was treated with trypsin, chymotrypsin, and CNBr, and the cleavage products were isolated and identified by HPLC and MS. Two unprecedented cleavage sites have been found. These fragments are valuable synthons in semi-synthesis to improve the chemical stability of this class of peptide-based antibiotics.

    5. Short KR-12 analogs designed from human cathelicidin LL-37 possessing both antimicrobial and antiendotoxic activities without mammalian cell toxicity (pages 700–707)

      Binu Jacob, Il-Seon Park, Jeong-Kyu Bang and Song Yub Shin

      Article first published online: 17 SEP 2013 | DOI: 10.1002/psc.2552

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      KR-12-a2, KR-12-a3 and KR-12-a4 showed much higher cell specificity and retained antiendotoxic activity as compared with parental LL-37. KR-12-a2, KR-12-a3, KR-12-a4 and KR-12-a5 have the potential for future development as a novel class of antimicrobial and anti-inflammatory therapeutic agents.

    6. Ethanol induced the formation of β-sheet and amyloid-like fibrils by surfactant-like peptide A6K (pages 708–716)

      Yongzhu Chen, Chengkang Tang, Zhihua Xing, Jie Zhang and Feng Qiu

      Article first published online: 17 SEP 2013 | DOI: 10.1002/psc.2553

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      Ethanol was used to induce conformational changes of the surfactant-like peptide A6K from unordered secondary structure to β-sheet, on the basis of which the self-assembling behavior of the peptide was changed to formation of amyloid-like fibrils.

    7. Identification of a pepducin acting as S1P3 receptor antagonist (pages 717–724)

      Beatrice Severino, Giuseppina Maria Incisivo, Ferdinando Fiorino, Antonio Bertolino, Francesco Frecentese, Francesco Barbato, Serena Manganelli, Giada Maggioni, Domenica Capasso, Giuseppe Caliendo, Vincenzo Santagada, Raffaella Sorrentino, Fiorentina Roviezzo and Elisa Perissutti

      Article first published online: 20 SEP 2013 | DOI: 10.1002/psc.2554

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      A novel peptidic S1P3 antagonist is described. Its ability to inhibit, in a dose-dependent manner, agonist-induced effects has been demonstrated in two different pharmacological models. The unique biological properties of the described antagonist make it a useful pharmacological tool for the study of S1P signaling involving the S1P3 receptor. Computational analysis suggested a correlation between specific conformations and activities.