‘Click’ chemistry synthesis and capillary electrophoresis study of 1,4-linked 1,2,3-triazole AZT-systemin conjugate (pages 696–703)
Michał Dobkowski, Aleksandra Szychowska, Małgorzata Pieszko, Anna Miszka, Monika Wojciechowska, Magdalena Alenowicz, Jarosław Ruczyński, Piotr Rekowski, Lech Celewicz, Jan Barciszewski and Piotr Mucha
Article first published online: 28 MAY 2014 | DOI: 10.1002/psc.2653
System (Sys), AVQSKPPSKRDPPKMQTD, is a defense peptide hormone involved in the wound response in tomato plant. To investigate whether Sys can transport cargo molecules through plant tissues, a conjugation of the peptide with 3′-azido-2′,3′-dideoxythymidine molecule using click chemistry was carried out. Capacity electrophoresis showed that click chemistry is a fast and efficient method of bioconjugation, and the conjugate is stable and easily translocated through tomato plant tissues.