CdSe/ZnS core-shell quantum dots (QDs) have been immobilized onto solid substrates by matrix assisted pulsed laser evaporation (MAPLE). An UV KrF* (λ = 248 nm, τFWHM ≅ 25 ns) excimer laser source was used for irradiations of the composite MAPLE targets. The targets were prepared by the dispersion of the CdSe/ZnS QDs in a solvent with high absorption at the incident laser radiation. The dependence of the surface morphology, crystalline structure, chemical composition, and functional properties of the laser transferred CdSe/ZnS QDs on the processing conditions as incident laser fluence value and ambient atmosphere inside the irradiation chamber was investigated. The possible physical mechanisms implied in the laser ablation process were identified.