Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor

Authors

  • Christina Huck,

    1. Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany
    2. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52525 Jülich, Germany
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    • These authors contributed equally to this work.
  • Johannes Schiffels,

    1. Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany
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    • These authors contributed equally to this work.
  • Cony N. Herrera,

    1. Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany
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  • Maximilian Schelden,

    1. Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany
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  • Thorsten Selmer,

    1. Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany
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  • Arshak Poghossian,

    1. Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany
    2. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52525 Jülich, Germany
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  • Marcus E. M. Baumann,

    1. Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany
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  • Patrick Wagner,

    1. Institute for Materials Research (IMO), Hasselt University, Wetenschapspark 1, 3590 Diepenbeek, Belgium
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  • Michael J. Schöning

    Corresponding author
    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52525 Jülich, Germany
    • Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany
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Abstract

Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.

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