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Original Paper
Long-term adsorption of fetal bovine serum on H/O-terminated diamond studied in situ by atomic force microscopy
Article first published online: 17 NOV 2009
DOI: 10.1002/pssb.200982257
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
physica status solidi (b)
Special Issue: Electronic Properties of Novel Materials (IWEPNM 2009)
Volume 246, Issue 11-12, pages 2832–2835, December 2009
Additional Information
How to Cite
Ukraintsev, E., Rezek, B., Kromka, A., Broz, A. and Kalbacova, M. (2009), Long-term adsorption of fetal bovine serum on H/O-terminated diamond studied in situ by atomic force microscopy. Phys. Status Solidi B, 246: 2832–2835. doi: 10.1002/pssb.200982257
Publication History
- Issue published online: 24 NOV 2009
- Article first published online: 17 NOV 2009
- Manuscript Accepted: 14 AUG 2009
- Manuscript Revised: 1 JUL 2009
- Manuscript Received: 23 APR 2009
Funded by
- AV0Z10100521
- KAN400100701 (GAAV)
- LC06040 (MŠMT)
- LC510 (MŠMT)
- MSM0021620806
- Abstract
- Article
- References
- Cited By
Keywords:
- 68.35.Np;
- 81.05.Uw;
- 87.14.E−;
- 87.17.Rt;
- 87.64.Dz;
- 87.85.J−
Abstract
We investigate adhesion of fetal bovine serum (FBS) on diamond surfaces which have alternating H/O-terminated surface patterns of 30 µm width prepared by hydrogen and oxygen plasma. The samples are immersed into 15% FBS in McCoy's 5A supporting medium and characterized by in situ atomic force microscopy (AFM) during 6 days of adsorption. Within the first day, 2–4 nm primary layer is formed on both H-/O-terminated surfaces. After 6 days, we observe 17 ± 5 nm FBS layer on O-terminated surface and 35 ± 5 nm layer on H-terminated surface. Adhesion of the primary and secondary layer is weaker on H-terminated surface. We present a model of FBS protein layers on H-/O-terminated diamond and we discuss implications for a preferential cell growth on O-terminated diamond. We also show that the preferential cell growth is not affected by the long-term FBS pre-adsorption.