1099-1573/asset/PTR_left.gif?v=1&s=61679c959d6ba2d597f752341613b4d11f86e770)
1099-1573/asset/PTR_right.gif?v=1&s=9ab10d74d00053b89129ee4003616901d34ded47)
Research Article
In vitro anti-Helicobacter pylori potential of methanol extract of Allium ascalonicum Linn. (Liliaceae) leaf: susceptibility and effect on urease activity
Article first published online: 26 MAY 2004
DOI: 10.1002/ptr.1265
Copyright © 2004 John Wiley & Sons, Ltd.
1099-1573/asset/cover.gif?v=1&s=6a64569c4c1b0de9f4868274faed96fd9265b457 "Phytotherapy Research")
Additional Information
How to Cite
Adeniyi, B. A. and Anyiam, F. M. (2004), In vitro anti-Helicobacter pylori potential of methanol extract of Allium ascalonicum Linn. (Liliaceae) leaf: susceptibility and effect on urease activity. Phytotherapy Research, 18: 358–361. doi: 10.1002/ptr.1265
Publication History
- Issue published online: 26 MAY 2004
- Article first published online: 26 MAY 2004
- Manuscript Accepted: 25 JUL 2002
- Manuscript Received: 13 DEC 2001
Funded by
- International Foundation for Science Sweden. Grant Number: F2884-1
- Abstract
- References
- Cited By
Keywords:
- Allium ascalonicum;
- Helicobacter pylori;
- urease activity;
- anti-Helicobacter pylori
Abstract
The crude methanol extract of the leaf of Allium ascalonicum was screened in vitro against five strains of Helicobacter pylori (Hp) (ATCC 24376, UCH 97001, UCH 97009, UCH 98026 and UCH 99039) for antibacterial activity by the agar diffusion method in Mueller-Hinton agar supplemented with defibrinated horse blood. All the strains were inhibited by the extract to varying degrees. The minimum inhibitory concentrations (MICs) of the extract against all the tested strains ranged from 6.25 to 12.5 mg/mL. The effects of increasing concentrations of the extract on the urease activity of three of the Helicobacter pylori strains were investigated further. The results showed that increasing the concentration of the extract decreased the urease activity of all the strains tested. Phytochemical screening of the plant showed that it contains alkaloids, cardiac glycosides and saponins. The anti-Hp activity observed is discussed in relation to the chemical constituents reportedly isolated from these plants and their traditional uses. The result of this work suggests that Allium ascalonicum has some therapeutic potential against Helicobacter pylori infection, which could be explored for patients with gastroduodenal disorders. Copyright © 2004 John Wiley & Sons, Ltd.