Supplementation with gliadin-combined plant superoxide dismutase extract promotes antioxidant defences and protects against oxidative stress

Authors

  • Ioannis Vouldoukis,

    1. ISOCELL Nutra SAS, 53 bd du Général Martial Valin, 75015, Paris, France
    2. INSERM U511, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, CHU-Pitié Salptérière Paris VI, 75013, Paris, France
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  • Marc Conti,

    1. INSERM U511, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, CHU-Pitié Salptérière Paris VI, 75013, Paris, France
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  • Pascal Krauss,

    1. ISOCELL Nutra SAS, 53 bd du Général Martial Valin, 75015, Paris, France
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  • Caroline Kamaté,

    1. ISOCELL Nutra SAS, 53 bd du Général Martial Valin, 75015, Paris, France
    2. INSERM U477, Hôpital Cochin, 75006 Paris, France
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  • Samantha Blazquez,

    1. INSERM U511, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, CHU-Pitié Salptérière Paris VI, 75013, Paris, France
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  • Maurel Tefit,

    1. INSERM U511, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, CHU-Pitié Salptérière Paris VI, 75013, Paris, France
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  • Dominique Mazier,

    1. INSERM U511, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, CHU-Pitié Salptérière Paris VI, 75013, Paris, France
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  • Alphonse Calenda,

    1. ISOCELL Nutra SAS, 53 bd du Général Martial Valin, 75015, Paris, France
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  • Bernard Dugas

    Corresponding author
    1. ISOCELL Nutra SAS, 53 bd du Général Martial Valin, 75015, Paris, France
    2. INSERM U511, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, CHU-Pitié Salptérière Paris VI, 75013, Paris, France
    • ISOCELL Nutra SAS, 53 blvd du Général Martial Valin, 75015, Paris, France.
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Abstract

The potential benefits to health of antioxidant enzymes supplied either through dietary intake or supplementation is still a matter of controversy. The development of dietary delivery systems using wheat gliadin biopolymers as a natural carrier represents a new alternative. Combination of antioxidant enzymes with this natural carrier not only delayed their degradation (i.e. the superoxide dismutase, SOD) during the gastrointestinal digestive process, but also promoted, in vivo, the cellular defences by strengthening the antioxidant status. The effects of supplementation for 28 days with a standardized melon SOD extract either combined (Glisodin®) or not with gliadin, were evaluated on various oxidative-stress biomarkers. As already described there was no change either in superoxide dismutase, catalase or glutathione peroxidase activities in blood circulation or in the liver following non-protected SOD supplementation. However, animals supplemented with Glisodin® showed a significant elevation in circulated antioxidant enzymes activities, correlated with an increased resistance of red blood cells to oxidative stress-induced hemolysis. In the presence of Sin-1, a chemical donor of peroxynitrites, mitochondria from hepatocytes regularly underwent membrane depolarization as the primary biological event of the apoptosis cascade. Hepatocytes isolated from animals supplemented with Glisodin® presented a delayed depolarization response and an enhanced resistance to oxidative stress-induced apoptosis. It is concluded that supplementation with gliadin-combined standardized melon SOD extract (Glisodin®) promoted the cellular antioxidant status and protected against oxidative stress-induced cell death. Copyright © 2004 John Wiley & Sons, Ltd.

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