Research Article

Supplementation with gliadin-combined plant superoxide dismutase extract promotes antioxidant defences and protects against oxidative stress

  1. Ioannis Vouldoukis1,2,
  2. Marc Conti2,
  3. Pascal Krauss1,
  4. Caroline Kamaté1,3,
  5. Samantha Blazquez2,
  6. Maurel Tefit2,
  7. Dominique Mazier2,
  8. Alphonse Calenda1,
  9. Bernard Dugas1,2,*

Article first published online: 1 MAR 2005

DOI: 10.1002/ptr.1542

Issue

Phytotherapy Research

Phytotherapy Research

Volume 18, Issue 12, pages 957–962, December 2004

Additional Information

How to Cite

Vouldoukis, I., Conti, M., Krauss, P., Kamaté, C., Blazquez, S., Tefit, M., Mazier, D., Calenda, A. and Dugas, B. (2004), Supplementation with gliadin-combined plant superoxide dismutase extract promotes antioxidant defences and protects against oxidative stress. Phytotherapy Research, 18: 957–962. doi: 10.1002/ptr.1542

Author Information

  1. 1

    ISOCELL Nutra SAS, 53 bd du Général Martial Valin, 75015, Paris, France

  2. 2

    INSERM U511, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, CHU-Pitié Salptérière Paris VI, 75013, Paris, France

  3. 3

    INSERM U477, Hôpital Cochin, 75006 Paris, France

*ISOCELL Nutra SAS, 53 blvd du Général Martial Valin, 75015, Paris, France.

Publication History

  1. Issue published online: 1 MAR 2005
  2. Article first published online: 1 MAR 2005
  3. Manuscript Accepted: 4 JUN 2004
  4. Manuscript Received: 14 MAY 2003

Funded by

  • CIFRE fellowship ISOCELL Pharma SAS.

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Keywords:

  • antioxidant;
  • plant superoxide dismutase;
  • gliadin;
  • oxidative stress

Abstract

The potential benefits to health of antioxidant enzymes supplied either through dietary intake or supplementation is still a matter of controversy. The development of dietary delivery systems using wheat gliadin biopolymers as a natural carrier represents a new alternative. Combination of antioxidant enzymes with this natural carrier not only delayed their degradation (i.e. the superoxide dismutase, SOD) during the gastrointestinal digestive process, but also promoted, in vivo, the cellular defences by strengthening the antioxidant status. The effects of supplementation for 28 days with a standardized melon SOD extract either combined (Glisodin®) or not with gliadin, were evaluated on various oxidative-stress biomarkers. As already described there was no change either in superoxide dismutase, catalase or glutathione peroxidase activities in blood circulation or in the liver following non-protected SOD supplementation. However, animals supplemented with Glisodin® showed a significant elevation in circulated antioxidant enzymes activities, correlated with an increased resistance of red blood cells to oxidative stress-induced hemolysis. In the presence of Sin-1, a chemical donor of peroxynitrites, mitochondria from hepatocytes regularly underwent membrane depolarization as the primary biological event of the apoptosis cascade. Hepatocytes isolated from animals supplemented with Glisodin® presented a delayed depolarization response and an enhanced resistance to oxidative stress-induced apoptosis. It is concluded that supplementation with gliadin-combined standardized melon SOD extract (Glisodin®) promoted the cellular antioxidant status and protected against oxidative stress-induced cell death. Copyright © 2004 John Wiley & Sons, Ltd.

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