An in vitro study of the protective effect of the flavonoid silydianin against reactive oxygen species

Authors

  • Małgorzata Zielińska-Przyjemska,

    Corresponding author
    1. Department of Pharmaceutical Biochemistry, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland
    • Department of Pharmaceutical Biochemistry, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland.
    Search for more papers by this author
  • Krzysztof Wiktorowicz

    1. Department of Biology and Environmental Sciences, Poznań University of Medical Sciences, Długa 1/2, 61-848 Poznań, Poland
    Search for more papers by this author

Abstract

The inhibitory effect of silydianin, an active constituent of Silybium marianum, on the in vitro production and release of oxidative products has been examined. Polymorphonuclear neutrophils (PMNs) play a primary role in the initiation and propagation of inflammatory responses. Their apoptosis is a major mechanism associated with the resolution of inflammatory reactions. Neutrophils were assessed for caspase-3 activity, the rst step in the execution phase of apoptosis. When cells were cultured with 100 µm silydianin for 24 h, caspase-3 was activated. Induction of apoptosis by silydianin was accompanied by a decrease in luminol-enhanced chemiluminescence as well as superoxide radical (O2•−) release in freshly isolated cells and lipid peroxidation in mouse spleen microsomes. No signicant effect of silydianin on PMN hydrogen peroxide production evaluated by a flow cytometric dichlorofluorescin oxidation assay was found. Such results indicate a possible antiinflammatory activity for silydianin, which regulates caspase-3 activation, affects cell membranes and acts as a free radical scavenger. Copyright © 2006 John Wiley & Sons, Ltd.

Ancillary