Modulation of selenite cataract by the flavonoid fraction of Emilia sonchifolia in experimental animal models
Article first published online: 28 SEP 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Volume 20, Issue 12, pages 1091–1095, December 2006
How to Cite
Lija, Y., Biju, P. G., Reeni, A., Cibin, T. R., Sahasranamam, V. and Abraham, A. (2006), Modulation of selenite cataract by the flavonoid fraction of Emilia sonchifolia in experimental animal models. Phytother. Res., 20: 1091–1095. doi: 10.1002/ptr.2005
- Issue published online: 22 NOV 2006
- Article first published online: 28 SEP 2006
- Manuscript Accepted: 13 AUG 2006
- Manuscript Revised: 10 AUG 2006
- Manuscript Received: 13 JAN 2006
- Kerala State Council for Science, Technology and Environment (KSCSTE), Government of Kerala, India. Grant Number: (T)17/R&D augmentation/04/KSCSTE
- Emilia sochifolia;
- oxidative stress;
The purpose of the study was to investigate the role of flavonoids from Emilia sonchifolia (ES) on the progression of selenite-induced cataract. The antioxidant property of the flavonoids isolated from ES was assessed by measuring its capacity to inhibit superoxide production and serum oxidation in vitro in comparison with quercetin. Based on these experiments, an in vivo study was conducted to evaluate the modulatory effects of the flavonoids against selenite cataract. Cataract was induced by a single subcutaneous injection of sodium selenite (4 mg/kg body weight). The treatment group received flavonoids from ES (1 mg/kg) and this was compared with the quercetin treated group. Lens opacification was monitored by a slit lamp microscope and classified into six stages. Activity of the antioxidant enzymes – superoxide dismutase and catalase – and the level of lipid peroxidation products thiobarbituric acid reacting substances and reduced glutathione were studied. Slit lamp examination showed that the flavonoid fraction from ES could modulate the progression of cataract. Activities of superoxide dismutase, catalase and reduced glutathione were found to be increased in the ES treated groups, while thiobarbituric acid reacting substances were decreased compared with the seleniteinduced group. The results suggest that flavonoids from ES can modulate lens opacification and oxidative stress in selenite-induced cataract. Copyright © 2006 John Wiley & Sons, Ltd.