Flavonoids, taxifolin and luteolin attenuate cellular melanogenesis despite increasing tyrosinase protein levels
Article first published online: 27 AUG 2008
Copyright © 2008 John Wiley & Sons, Ltd.
Volume 22, Issue 9, pages 1200–1207, September 2008
How to Cite
An, S. M., Kim, H. J., Kim, J.-E. and Boo, Y. C. (2008), Flavonoids, taxifolin and luteolin attenuate cellular melanogenesis despite increasing tyrosinase protein levels. Phytother. Res., 22: 1200–1207. doi: 10.1002/ptr.2435
- Issue published online: 27 AUG 2008
- Article first published online: 27 AUG 2008
- Manuscript Accepted: 15 OCT 2007
- Manuscript Revised: 28 AUG 2007
- Manuscript Received: 26 APR 2007
- Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea. Grant Number: A06-00043030
- Brain Korea 21 Project in 2006
- taxifolin, luteolin
Flavonoids are a group of polyphenolic compounds widely distributed in plants. Their potent bio-activities and relatively low toxicity have rendered them useful ingredients in functional cosmetics. The purpose of the present study was to examine their potential effects on cellular melanogenesis. When tested in murine melanoma B16F10 cells activated by α-melanocyte stimulating hormone (α-MSH), taxifolin and luteolin inhibited the cellular melanogenesis as effectively as arbutin, one of the most widely used hypopigmenting agents in cosmetics. As opposed to their antimelanogenic effects, taxifolin and luteolin rather increased the tyrosinase protein levels in the absence and presence of α-MSH. However, these flavonoids effectively inhibited tyrosinase-catalysed oxidation of l-dihydroxyphenylalanine in cell-free extracts and in living cells. Furthermore, they attenuated cell pigmentation induced by expression of exogenous human tyrosinase. Therefore, the antimelanogenic effects of taxifolin and luteolin are attributed to their inhibitory effects on tyrosinase enzymatic activity, despite their effects on increasing tyrosinase protein levels. Copyright © 2008 John Wiley & Sons, Ltd.