Research Article

Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities

  1. Muhammad Shahid1,
  2. Muhammad Tayyab1,
  3. Farah Naz1,
  4. Amer Jamil1,
  5. Muhammad Ashraf2,
  6. Anwarul Hassan Gilani3,*

Article first published online: 9 OCT 2008

DOI: 10.1002/ptr.2544

Issue

Phytotherapy Research

Phytotherapy Research

Volume 22, Issue 12, pages 1646–1649, December 2008

Additional Information

How to Cite

Shahid, M., Tayyab, M., Naz, F., Jamil, A., Ashraf, M. and Gilani, A. H. (2008), Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities. Phytother. Res., 22: 1646–1649. doi: 10.1002/ptr.2544

Author Information

  1. 1

    Molecular Biochemistry Lab., Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan

  2. 2

    Department of Botany, University of Agriculture, Faisalabad-38040, Pakistan

  3. 3

    Natural Products Research Division, Department of Biological and Biomedical Science, Aga Khan University Medical College, Karachi, Pakistan

*Natural Products Research Division, Department of Biological and Biomedical Science, Aga Khan University Medical College, Karachi, Pakistan.

Publication History

  1. Issue published online: 8 DEC 2008
  2. Article first published online: 9 OCT 2008
  3. Manuscript Accepted: 28 JAN 2008
  4. Manuscript Revised: 23 JAN 2008
  5. Manuscript Received: 5 SEP 2007

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Keywords:

  • Croton tiglium;
  • bioactive peptide/protein;
  • antibacterial;
  • antifungal

Abstract

This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH4)2SO4 precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity. Copyright © 2008 John Wiley & Sons, Ltd.

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