Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities
Article first published online: 9 OCT 2008
Copyright © 2008 John Wiley & Sons, Ltd.
Volume 22, Issue 12, pages 1646–1649, December 2008
How to Cite
Shahid, M., Tayyab, M., Naz, F., Jamil, A., Ashraf, M. and Gilani, A. H. (2008), Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities. Phytother. Res., 22: 1646–1649. doi: 10.1002/ptr.2544
- Issue published online: 8 DEC 2008
- Article first published online: 9 OCT 2008
- Manuscript Accepted: 28 JAN 2008
- Manuscript Revised: 23 JAN 2008
- Manuscript Received: 5 SEP 2007
- Croton tiglium;
- bioactive peptide/protein;
This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH4)2SO4 precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity. Copyright © 2008 John Wiley & Sons, Ltd.