Experiments were carried out to determine the efficacy of the Mikania cordata root extract in the tissue repair activities in mice intoxicated with carbon tetrachloride (CCl4). We have estimated total plasma protein, albumin, globulin, blood urea, hepatic microsomal ribonucleic acid (RNA) and cytochrome P-450 level. Acute single intraperitoneal administration of CCl4 (1 mL of 20% v/v in olive oil/kg body weight) decreased albumin and increased globulin level and thereby reduced the albumin-globulin ratio. A lower blood urea level was also noticed after treatment of mice with CCl4. A 1 h pretreatment with the root extract tended to reverse these features which were found to be dose-dependent, but the results were statistically significant in the dose range of 100–150mg/kg. The M. cordata root extract (at 150mg/kg) dramatically improved the level of hepatic microsomal RNA (42.2%; p<0.001) and cytochrome P-450 content (70.2%; p<0.001) that were altered in CCl4-induced liver damage. Based on these observations, it is considered that M. cordata root extract may alleviate the deleterious effects of CCl4, protect the liver cells and activate the hepatic reticuloendothelial system-mediated defence mechanism as well as the regeneration of protein synthesis.