Ji-hoon Kim and Yun-chang Koo have contributed equally this work and should be considered co-first authors.
Mutagenicity and Oral Toxicity Studies of Terminalia chebula
Article first published online: 2 MAY 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 1, pages 39–47, January 2012
How to Cite
Kim, J.-h., Koo, Y.-c., Hong, C.-O., Yang, S.-Y., Jun, W. and Lee, K.-W. (2012), Mutagenicity and Oral Toxicity Studies of Terminalia chebula. Phytother. Res., 26: 39–47. doi: 10.1002/ptr.3504
- Issue published online: 6 JAN 2012
- Article first published online: 2 MAY 2011
- Manuscript Accepted: 18 MAR 2011
- Manuscript Revised: 17 MAR 2011
- Manuscript Received: 12 SEP 2010
- Terminalia chebula;
- chebulic acid;
- oral toxicity;
- bacterial reverse mutation assay
The fruit of Terminalia chebula Retz. (T. chebula), which is a member of the Combfreetaceae family, is used widely in Asian countries as a traditional folk medicine, and its extract has been reported to be an anticancer, antidiabetic and anticaries agent. In our previous study, chebulic acid isolated from T. chebula extract was confirmed to show antioxidant activity and protective action against endothelial cell dysfunction. In order to support the safety-in-use of the ethyl acetate (EtOAc)-soluble portion of a T. chebula ethanol extract containing 29.4% chebulic acid content, the prepared portion was tested in an in vitro mutagenicity assay, and a single- and 14-day repeated dose oral toxicity study. In the bacterial mutation assay, up to 5000 µg/mL concentration of the EtOAc-soluble portion, the numbers of colonies did not increase whether with or without metabolic activation. In the oral toxicity study, the single oral dose of the extract at 2000 mg/kg did not produce mortality or abnormal lesions in the internal organs of rats. The results of a 14-day orally repeated dose showed that the EtOAc-soluble portion of T. chebula ethanol extracts gave no adverse effects at dosages of 2000 mg/kg in rats in the study. Copyright © 2011 John Wiley & Sons, Ltd.