Get access

Mutagenicity and Oral Toxicity Studies of Terminalia chebula

Authors

  • Ji-hoon Kim,

    1. Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
    Search for more papers by this author
    • Ji-hoon Kim and Yun-chang Koo have contributed equally this work and should be considered co-first authors.
  • Yun-chang Koo,

    1. Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
    Search for more papers by this author
    • Ji-hoon Kim and Yun-chang Koo have contributed equally this work and should be considered co-first authors.
  • Chung-Oui Hong,

    1. Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
    Search for more papers by this author
  • Sung-Yong Yang,

    1. Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
    Search for more papers by this author
  • Woojin Jun,

    1. Department of Food and Nutrition, Chonnam National University, Gwangju, Korea
    Search for more papers by this author
  • Kwang-Won Lee

    Corresponding author
    • Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
    Search for more papers by this author

Kwang-Won Lee, Department of Food Science, College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Korea.

E-mail: kwangwon@korea.ac.kr

Abstract

The fruit of Terminalia chebula Retz. (T. chebula), which is a member of the Combfreetaceae family, is used widely in Asian countries as a traditional folk medicine, and its extract has been reported to be an anticancer, antidiabetic and anticaries agent. In our previous study, chebulic acid isolated from T. chebula extract was confirmed to show antioxidant activity and protective action against endothelial cell dysfunction. In order to support the safety-in-use of the ethyl acetate (EtOAc)-soluble portion of a T. chebula ethanol extract containing 29.4% chebulic acid content, the prepared portion was tested in an in vitro mutagenicity assay, and a single- and 14-day repeated dose oral toxicity study. In the bacterial mutation assay, up to 5000 µg/mL concentration of the EtOAc-soluble portion, the numbers of colonies did not increase whether with or without metabolic activation. In the oral toxicity study, the single oral dose of the extract at 2000 mg/kg did not produce mortality or abnormal lesions in the internal organs of rats. The results of a 14-day orally repeated dose showed that the EtOAc-soluble portion of T. chebula ethanol extracts gave no adverse effects at dosages of 2000 mg/kg in rats in the study. Copyright © 2011 John Wiley & Sons, Ltd.

Ancillary