These authors contributed equally to this work.
Mechanistic Studies on the Antidiabetic Activity of a Polysaccharide-rich Extract of Radix Ophiopogonis
Article first published online: 11 MAY 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 26, Issue 1, pages 101–105, January 2012
How to Cite
Ding, L., Li, P., Lau, C. B. S., Chan, Y. W., Xu, D., Fung, K.-P. and Su, W. (2012), Mechanistic Studies on the Antidiabetic Activity of a Polysaccharide-rich Extract of Radix Ophiopogonis. Phytother. Res., 26: 101–105. doi: 10.1002/ptr.3505
- Issue published online: 6 JAN 2012
- Article first published online: 11 MAY 2011
- Manuscript Accepted: 15 MAR 2011
- Manuscript Revised: 22 JAN 2011
- Manuscript Received: 17 JUL 2010
- Radix Ophiopogonis;
- hypoglycemic mechanism;
- diabetes mellitus;
To study the hypoglycemic mechanisms of a polysaccharide-rich extract of Radix Ophiopogonis, the influences of the extract on activity of NIT-1 insulinoma cells damaged by streptozotocin (STZ), activity of α-glucosidase, glucose absorption into intestinal brush border membrane vesicles, gluconeogenesis by H4IIE hepatoma cells and glucose uptake by 3T3-L1 adipocytes were investigated. The results show that the extract improved the activity of NIT-1 cells damaged by STZ, inhibited glucose absorption into intestinal brush border membrane vesicles and reduced the activity of α-glucosidase. However, gluconeogenesis in H4IIE cells and glucose uptake in 3T3-L1 adipocytes did not change significantly in the presence of the extract. These results suggest that the hypoglycemic mechanisms of the polysaccharide-rich extract of Radix Ophiopogonis are caused by protection in pancreatic islet cells and the inhibition of carbohydrate digestion and absorption. This is possibly the first report on the underlying mechanisms responsible for the antidiabetic effect of Radix Ophiopogonis. Copyright © 2011 John Wiley & Sons, Ltd.